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Applied and Environmental Microbiology, April 2001, p. 1581-1586, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1581-1586.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genomic Relatedness within Five Common Finnish Campylobacter jejuni Pulsed-Field Gel Electrophoresis Genotypes Studied by Amplified Fragment Length Polymorphism Analysis, Ribotyping, and Serotyping

Marja-Liisa Hänninen,1,* Päivikki Perko-Mäkelä,1 Hilpi Rautelin,2,3 Birgitta Duim,4 and Jaap A. Wagenaar4

Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine,1 and the Department of Bacteriology and Immunology, Haartman Institute,2 University of Helsinki, and Helsinki University Central Hospital Diagnostics,3 00014 University of Helsinki, Finland, and Department of Bacteriology, Institute for Animal Science and Health, Lelystad, The Netherlands4

Received 13 April 2000/Accepted 6 September 2000

Thirty-five Finnish Campylobacter jejuni strains with five SmaI/SacII pulsed-field gel electrophoresis (PFGE) genotypes selected among human and chicken isolates from 1997 and 1998 were used for comparison of their PFGE patterns, amplified fragment length polymorphism (AFLP) patterns, HaeIII ribotypes, and heat-stable (HS) serotypes. The discriminatory power of PFGE, AFLP, and ribotyping with HaeIII were shown to be at the same level for this selected set of strains, and these methods assigned the strains into the same groups. The PFGE and AFLP patterns within a genotype were highly similar, indicating genetic relatedness. The same HS serotypes were distributed among different genotypes, and different serotypes were identified within one genotype. HS serotype 12 was only associated with the combined genotype G1 (PFGE-AFLP-ribotype). These studies using polyphasic genotyping methods suggested that common Finnish C. jejuni genotypes form genetic lineages which colonize both humans and chickens.

* Corresponding author. Mailing address: Faculty of Veterinary Medicine, Department of Food and Environmental Hygiene, P.O. Box 57, FIN-00014 University of Helsinki, Finland. Phone: 358-9-19149704. Fax: 358-9-19149718. E-mail: marja-liisa.hanninen{at}helsinki.fi.

Applied and Environmental Microbiology, April 2001, p. 1581-1586, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1581-1586.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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