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Applied and Environmental Microbiology, April 2001, p. 1607-1612, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1607-1612.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Regulation of Fumonisin B1 Biosynthesis and Conidiation in Fusarium verticillioides by a Cyclin-Like (C-Type) Gene, FCC1†

Won-Bo Shim and Charles P. Woloshuk*

Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907

Received 7 September 2000/Accepted 25 January 2001

Fumonisins are a group of mycotoxins produced in corn kernels by the plant-pathogenic fungus Fusarium verticillioides. A mutant of the fungus, FT536, carrying a disrupted gene named FCC1 (for Fusarium cyclin C1) resulting in altered fumonisin B1 biosynthesis was generated. FCC1 contains an open reading frame of 1,018 bp, with one intron, and encodes a putative 319-amino-acid polypeptide. This protein is similar to UME3 (also called SRB11 or SSN8), a cyclin C of Saccharomyces cerevisiae, and contains three conserved motifs: a cyclin box, a PEST-rich region, and a destruction box. Also similar to the case for C-type cyclins, FCC1 was constitutively expressed during growth. When strain FT536 was grown on corn kernels or on defined minimal medium at pH 6, conidiation was reduced and FUM5, the polyketide synthase gene involved in fumonisin B1 biosynthesis, was not expressed. However, when the mutant was grown on a defined minimal medium at pH 3, conidiation was restored, and the blocks in expression of FUM5 and fumonisin B1 production were suppressed. Our data suggest that FCC1 plays an important role in signal transduction regulating secondary metabolism (fumonisin biosynthesis) and fungal development (conidiation) in F. verticillioides.

* Corresponding author. Mailing address: Department of Botany & Plant Pathology, Purdue University, 1155 Lilly Hall, West Lafayette, IN 47907. Phone: (765) 494-3450. Fax: (765) 494-0363. E-mail: woloshuk{at}btny.purdue.edu.

dagger Journal publication 16348 of the Purdue University Agricultural Research Program.

Applied and Environmental Microbiology, April 2001, p. 1607-1612, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1607-1612.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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