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Applied and Environmental Microbiology, April 2001, p. 1619-1627, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1619-1627.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Prevalence, Antibiotic Susceptibility, and Diversity of Escherichia coli O157:H7 Isolates from a Longitudinal Study of Beef Cattle Feedlots†

John C. Galland,1,* Doreene R. Hyatt,1,Dagger Scott S. Crupper,1,§ and David W. Acheson2

Food Animal Health and Management Center, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506,1 and Division of Infectious Diseases, New England Medical Center, Boston, Massachusetts 021112

Received 14 September 2000/Accepted 16 January 2001

Prevalence, antibiotic susceptibility, and genetic diversity were determined for Escherichia coli O157:H7 isolated over 11 months from four beef cattle feedlots in southwest Kansas. From the fecal pat (17,050) and environmental (7,134) samples collected, 57 isolates of E. coli O157:H7 were identified by use of bacterial culture and latex agglutination (C/LA). PCR showed that 26 isolates were eaeA gene positive. Escherichia coli O157:H7 was identified in at least one of the four feedlots in 14 of the 16 collections by C/LA and in 9 of 16 collections by PCR, but consecutive positive collections at a single feedlot were rare. Overall prevalence in fecal pat samples was low (0.26% by C/LA, and 0.08% by PCR). No detectable differences in prevalence or antibiotic resistance were found between isolates collected from home pens and those from hospital pens, where antibiotic use is high. Resistant isolates were found for six of the eight antibiotics that could be used to treat E. coli infections in food animals, but few isolates were multidrug resistant. The high diversity of isolates as measured by random amplification of polymorphic DNA and other characteristics indicates that the majority of isolates were unique and did not persist at a feedlot, but probably originated from incoming cattle. The most surprising finding was the low frequency of virulence markers among E. coli isolates identified initially by C/LA as E. coli O157:H7. These results demonstrate that better ways of screening and confirming E. coli O157:H7 isolates are required for accurate determination of prevalence.

* Corresponding author. Mailing address: Food Animal Health and Management Center, College of Veterinary Medicine, Kansas State University, 1800 Denison Ave., Manhattan, KS 66506. Phone: (785) 532-4262. Fax: (785) 532-4288. E-mail: galland{at}vet.ksu.edu.

dagger This is contribution no. 01-108-J from the Kansas Agricultural Experiment Station.

Dagger Present address: Department of Microbiology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.

§ Present address: Division of Biological Sciences, Emporia State University, Emporia, KS 66801.

Applied and Environmental Microbiology, April 2001, p. 1619-1627, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1619-1627.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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