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Applied and Environmental Microbiology, April 2001, p. 1744-1750, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1744-1750.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Novel alpha -Amylase That Is Highly Resistant to Chelating Reagents and Chemical Oxidants from the Alkaliphilic Bacillus Isolate KSM-K38

Hiroshi Hagihara,* Kazuaki Igarashi, Yasuhiro Hayashi, Keiji Endo, Kaori Ikawa-Kitayama, Katsuya Ozaki, Shuji Kawai, and Susumu Ito

Tochigi Research Laboratories of Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan

Received 9 October 2000/Accepted 2 February 2001

A novel alpha -amylase (AmyK38) was found in cultures of an alkaliphilic Bacillus isolate designated KSM-K38. Based on the morphological and physiological characteristics and phylogenetic position as determined by 16S ribosomal DNA gene sequencing and DNA-DNA reassociation analysis, it was suggested that the isolate was a new species of the genus Bacillus. The enzyme had an optimal pH of 8.0 to 9.5 and displayed maximum catalytic activity at 55 to 60°C. The apparent molecular mass was approximately 55 kDa, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the isoelectric point was around pH 4.2. This enzyme efficiently hydrolyzed various carbohydrates to yield maltotriose, maltohexaose, maltoheptaose, and, in addition, maltose as major end products after completion of the reaction. The activity was not prevented at all by EDTA and EGTA at concentrations as high as 100 mM. Moreover, AmyK38 was highly resistant to chemical oxidation and maintained more than 80% of its original activity even after incubation for 1 h in the presence of excess H2O2 (1.8 M).

* Corresponding author. Mailing address: Tochigi Research Laboratories of Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan. Phone: 81 (285) 68-7516. Fax: 81 (285) 68-7547. E-mail: 400297{at}kastanet.kao.co.jp.

Applied and Environmental Microbiology, April 2001, p. 1744-1750, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1744-1750.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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