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Applied and Environmental Microbiology, April 2001, p. 1935-1939, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1935-1939.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

16S Ribosomal DNA Terminal Restriction Fragment Pattern Analysis of Bacterial Communities in Feces of Rats Fed Lactobacillus acidophilus NCFM

Christopher W. Kaplan,1 Johanna C. Astaire,1 Mary Ellen Sanders,2 Bandaru S. Reddy,3 and Christopher L. Kitts1,*

Environmental Biotechnology Institute1 and Dairy Products Technology Center,2 California Polytechnic State University, San Luis Obispo, California 93407, and American Health Foundation, Valhalla, New York 105953

Received 16 August 2000/Accepted 6 January 2001

16S ribosomal DNA terminal restriction fragment patterns from rat fecal samples were analyzed to track the dynamics of Lactobacillus acidophilus NCFM and discern bacterial populations that changed during feeding with NCFM. Lactobacillus johnsonii and Ruminococcus flavefaciens were tentatively identified as such bacterial populations. The presence of L. johnsonii was confirmed by isolation from feces.

* Corresponding author. Mailing address: Environmental Biotechnology Institute, California Polytechnic State University, San Luis Obispo, CA 93407. Phone: (805) 756-2949. Fax: (805) 756-1419. E-mail: ckitts{at}calpoly.edu.

Applied and Environmental Microbiology, April 2001, p. 1935-1939, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1935-1939.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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