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Applied and Environmental Microbiology, April 2001, p. 1940-1944, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1940-1944.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genotypic Analysis of Escherichia coli Strains from Poultry Carcasses and Their Susceptibilities to Antimicrobial Agents

Ifigenia Geornaras,1 John W. Hastings,2 and Alexander von Holy1,*

Department of Molecular and Cell Biology, University of the Witwatersrand, Wits 2050,1 and School of Molecular and Cellular Biosciences, University of Natal, Scottsville 3209,2 South Africa

Received 9 August 2000/Accepted 3 January 2001

Plasmid profiling and amplified fragment length polymorphism (AFLP) analysis were used to genotype 50 Escherichia coli strains from poultry carcasses. Thirty different plasmid profiles were evident, and clustering of the AFLP data showed that they were a distinctly heterogeneous group of strains. Susceptibility testing against five antimicrobial agents used in the South African poultry industry showed all strains to be susceptible to danofloxacin and colistin, while the majority (96%) were resistant to two tetracyclines.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, Wits 2050, South Africa. Phone: 27 11 717 6374. Fax: 27 11 339 7377. E-mail: alex{at}gecko.biol.wits.ac.za.

Applied and Environmental Microbiology, April 2001, p. 1940-1944, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1940-1944.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.