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Applied and Environmental Microbiology, May 2001, p. 2360-2364, Vol. 67, No. 5
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.5.2360-2364.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Differentiation of Environmental and Clinical Isolates of Vibrio mimicus from Vibrio cholerae by Multilocus Enzyme Electrophoresis

Verônica V. Vieira,1,2,* Luiz Fernando M. Teixeira,2,3 Ana Carolina P. Vicente,2 Hooman Momen,1 and Carlos André Salles1

Department of Biochemistry and Molecular Biology1 and Department of Genetics,2 Instituto Oswaldo Cruz, Rio de Janeiro, and Department of Clinical Analysis, Universidade Federal de Ouro Preto, Minas Gerais,3 Brazil

Received 6 October 2000/Accepted 20 February 2001

In this study, we demonstrated that analyzed strains of Vibrio mimicus and Vibrio cholerae could be separated in two groups by using multilocus enzyme electrophoresis (MEE) data from 14 loci. We also showed that the combination of four enzymatic loci enables us to differentiate these two species. Our results showed that the ribosomal intergenic spacer regions PCR-mediated identification system failed, in some cases, to differentiate between V. mimicus and V. cholerae. On the other hand, MEE proved to be a powerful molecular tool for the discrimination of these two species even when atypical strains were analyzed.

* Corresponding author. Mailing address: Departamento de Genética, Fundação Oswaldo Cruz, Avenida Brasil 4365, Rio de Janeiro, Brazil CEP 21045-900. Phone: 55-21-5984242. Fax: 55-21-2694282. E-mail: vvieira{at}gene.dbbm.fiocruz.br.

Applied and Environmental Microbiology, May 2001, p. 2360-2364, Vol. 67, No. 5
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.5.2360-2364.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Thompson, C. C., Thompson, F. L., Vicente, A. C. P. (2008). Identification of Vibrio cholerae and Vibrio mimicus by multilocus sequence analysis (MLSA). Int. J. Syst. Evol. Microbiol. 58: 617-621 [Abstract] [Full Text]  
  • Thompson, F. L., Iida, T., Swings, J. (2004). Biodiversity of Vibrios. Microbiol. Mol. Biol. Rev. 68: 403-431 [Abstract] [Full Text]  
  • Thompson, F. L., Thompson, C. C., Vicente, A. C. P., Theophilo, G. N. D., Hofer, E., Swings, J. (2003). Genomic Diversity of Clinical and Environmental Vibrio cholerae Strains Isolated in Brazil between 1991 and 2001 as Revealed by Fluorescent Amplified Fragment Length Polymorphism Analysis. J. Clin. Microbiol. 41: 1946-1950 [Abstract] [Full Text]  


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