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Applied and Environmental Microbiology, May 2001, p. 2367-2370, Vol. 67, No. 5
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.5.2367-2370.2001

Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7

Gaylen A. Uhlich,* James E. Keen, and Robert O. Elderdagger

Roman L. Hruska U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933

Received 9 October 2000/Accepted 16 February 2001

Single-base-pair csgD promoter mutations in human outbreak Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 coincided with differential Congo red dye binding from curli fiber expression. Red phenotype csgD::lacZ promoter fusions had fourfold-greater expression than white promoter fusions. Cloning the red variant csgDEFG operon into white variants induced the red phenotype. Substrate utilization differed between red and white variants.

* Corresponding author. Present address: USDA, ARS, Eastern Regional Research Center, 600 East Mermaid Ln., Wyndmoor, PA 19038. Phone: (215) 233-6740. Fax: (215) 233-6581. E-mail: guhlich{at}arserrc.gov.

dagger Present address: USDA, ARS, Southern Plains Agricultural Research Center, College Station, TX 77845.

Applied and Environmental Microbiology, May 2001, p. 2367-2370, Vol. 67, No. 5
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.5.2367-2370.2001


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