Applied and Environmental Microbiology, June 2001, p. 2395-2403, Vol. 67, No. 6
Max Planck Institute for Terrestrial
Microbiology, D-35043 Marburg, Germany
Received 31 October 2000/Accepted 9 March 2001
The activity and community structure of methanotrophs in
compartmented microcosms were investigated over the growth period of
rice plants. In situ methane oxidation was important only during the
vegetative growth phase of the plants and later became negligible. The
in situ activity was not directly correlated with methanotrophic cell
counts, which increased even after the decrease in in situ activity,
possibly due to the presence of both vegetative cells and resting
stages. By dividing the microcosms into two soil and two root
compartments it was possible to locate methanotrophic growth and
activity, which was greatest in the rhizoplane of the rice plants.
Molecular analysis by denaturing gradient gel electrophoresis and
fluorescent in situ hybridization (FISH) with family-specific probes
revealed the presence of both families of methanotrophs in soil and
root compartments over the whole season. Changes in community structure
were detected only for members of the Methylococcaceae and
could be associated only with changes in the genus
Methylobacter and not with changes in the dominance of
different genera in the family Methylococcaceae. For the
family Methylocystaceae stable communities in all
compartments for the whole season were observed. FISH analysis revealed
evidence of in situ dominance of the Methylocystaceae in
all compartments. The numbers of Methylococcaceae cells
were relatively high only in the rhizoplane, demonstrating the
importance of rice roots for growth and maintenance of methanotrophic
diversity in the soil.
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.6.2395-2403.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Changes in Activity and Community Structure of
Methane-Oxidizing Bacteria over the Growth Period of Rice
*
Corresponding author. Mailing address: Max Planck
Institute for Terrestrial Microbiology, Karl-von-Frisch Strasse,
D-35043 Marburg, Germany. Phone: 49-(0)6421-178 820. Fax:
49-(0)6421-178 809. E-mail:
frenzel{at}mailer.uni-marburg.de.
Applied and Environmental Microbiology, June 2001, p. 2395-2403, Vol. 67, No. 6
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.6.2395-2403.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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