This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Monpoeho, S.
Right arrow Articles by Ferré, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Monpoeho, S.
Right arrow Articles by Ferré, V.
Agricola
Right arrow Articles by Monpoeho, S.
Right arrow Articles by Ferré, V.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2001, p. 2484-2488, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2484-2488.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Best Viral Elution Method Available for Quantification of Enteroviruses in Sludge by Both Cell Culture and Reverse Transcription-PCR

S. Monpoeho,1 A. Maul,2 B. Mignotte-Cadiergues,3 L. Schwartzbrod,3 S. Billaudel,1 and V. Ferré1,*

Laboratoire de Virologie, CHU Hotel Dieu, Nantes,1 I.U.T, Department of Statistics and Data Processing, Metz,2 and LCPME-Virology, Faculty of Pharmacy, Nancy,3 France

Received 4 January 2001/Accepted 21 March 2001

The aim of this study was to select one or several virus extraction techniques that enable simultaneous detection of enterovirus genomes and infectious particles in different types of urban sludge. Eight techniques were compared by using 16 different liquid and solid sludge samples. The numbers of infectious enteroviruses in cell cultures were determined by using the most-probable-number method. The enterovirus genome was quantified by a single-tube reverse transcription-PCR using TaqMan technology. The results were statistically analyzed by Friedman's test, a nonparametric test for analysis of randomized block data using only ranks in terms of extraction technique efficiency. Two techniques seemed to yield higher viral titers as determined by simultaneous detection by cell culture and PCR. The first involved a 10% beef extract solution at pH 9 and sonication; the second involved a 0.3 M NaCl-7% beef extract solution at pH 7.5 followed by Freon treatment. In solid sludge, no significant differences were observed among the eight techniques tested. Both of the best techniques can be used for simultaneous detection of infectious enterovirus particles and genomes in any type of urban sludge.

* Corresponding author. Mailing address: Laboratoire de Virologie, CHU Hotel Dieu, 9 quai moncousu, 44035 Nantes, France. Phone: (33)-2-40084101. Fax: (33)-2-40084139. E-mail: sbillaudel{at}chu-nantes.fr.

Applied and Environmental Microbiology, June 2001, p. 2484-2488, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2484-2488.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Viau, E., Peccia, J. (2009). Survey of Wastewater Indicators and Human Pathogen Genomes in Biosolids Produced by Class A and Class B Stabilization Treatments. Appl. Environ. Microbiol. 75: 164-174 [Abstract] [Full Text]  
  • Guan, J., Chan, M., Ma, B., Grenier, C., Wilkie, D. C., Pasick, J., Brooks, B. W., Spencer, J. L. (2008). Development of Methods for Detection and Quantification of Avian Influenza and Newcastle Disease Viruses in Compost by Real-Time Reverse Transcription Polymerase Chain Reaction and Virus Isolation. Poult. Sci. 87: 838-843 [Abstract] [Full Text]  
  • Helmi, K., Skraber, S., Gantzer, C., Willame, R., Hoffmann, L., Cauchie, H.-M. (2008). Interactions of Cryptosporidium parvum, Giardia lamblia, Vaccinal Poliovirus Type 1, and Bacteriophages {phi}X174 and MS2 with a Drinking Water Biofilm and a Wastewater Biofilm. Appl. Environ. Microbiol. 74: 2079-2088 [Abstract] [Full Text]  
  • Noble, R. T., Griffith, J. F., Blackwood, A. D., Fuhrman, J. A., Gregory, J. B., Hernandez, X., Liang, X., Bera, A. A., Schiff, K. (2006). Multitiered Approach Using Quantitative PCR To Track Sources of Fecal Pollution Affecting Santa Monica Bay, California. Appl. Environ. Microbiol. 72: 1604-1612 [Abstract] [Full Text]  
  • Fuhrman, J. A., Liang, X., Noble, R. T. (2005). Rapid Detection of Enteroviruses in Small Volumes of Natural Waters by Real-Time Quantitative Reverse Transcriptase PCR. Appl. Environ. Microbiol. 71: 4523-4530 [Abstract] [Full Text]  
  • Williamson, K. E., Radosevich, M., Wommack, K. E. (2005). Abundance and Diversity of Viruses in Six Delaware Soils. Appl. Environ. Microbiol. 71: 3119-3125 [Abstract] [Full Text]  
  • Fong, T.-T., Lipp, E. K. (2005). Enteric Viruses of Humans and Animals in Aquatic Environments: Health Risks, Detection, and Potential Water Quality Assessment Tools. Microbiol. Mol. Biol. Rev. 69: 357-371 [Abstract] [Full Text]  
  • Monpoeho, S., Maul, A., Bonnin, C., Patria, L., Ranarijaona, S., Billaudel, S., Ferre, V. (2004). Clearance of Human-Pathogenic Viruses from Sludge: Study of Four Stabilization Processes by Real-Time Reverse Transcription-PCR and Cell Culture. Appl. Environ. Microbiol. 70: 5434-5440 [Abstract] [Full Text]  
  • Williamson, K. E., Wommack, K. E., Radosevich, M. (2003). Sampling Natural Viral Communities from Soil for Culture-Independent Analyses. Appl. Environ. Microbiol. 69: 6628-6633 [Abstract] [Full Text]  
  • Gassilloud, B., Schwartzbrod, L., Gantzer, C. (2003). Presence of Viral Genomes in Mineral Water: a Sufficient Condition To Assume Infectious Risk?. Appl. Environ. Microbiol. 69: 3965-3969 [Abstract] [Full Text]  
  • Costa-Mattioli, M., Cristina, J., Romero, H., Perez-Bercof, R., Casane, D., Colina, R., Garcia, L., Vega, I., Glikman, G., Romanowsky, V., Castello, A., Nicand, E., Gassin, M., Billaudel, S., Ferre, V. (2002). Molecular Evolution of Hepatitis A Virus: a New Classification Based on the Complete VP1 Protein. J. Virol. 76: 9516-9525 [Abstract] [Full Text]  
  • Mackay, I. M., Arden, K. E., Nitsche, A. (2002). Real-time PCR in virology. Nucleic Acids Res 30: 1292-1305 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»