This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Logan, B. E.
Right arrow Articles by Unz, R. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Logan, B. E.
Right arrow Articles by Unz, R. F.
Agricola
Right arrow Articles by Logan, B. E.
Right arrow Articles by Unz, R. F.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2001, p. 2499-2506, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2499-2506.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Kinetics of Perchlorate- and Chlorate-Respiring Bacteria

Bruce E. Logan,1,* Husen Zhang,1 Peter Mulvaney,1 Michael G. Milner,2,3 Ian M. Head,2,3 and Richard F. Unz1

Department of Civil and Environmental Engineering, The Pennsylvania State University, University Park, Pennsylvania,1 and Fossil Fuels & Environmental Geochemistry,2 and Centre for Molecular Ecology,3 University of Newcastle, Newcastle upon Tyne, United Kingdom

Received 30 November 2000/Accepted 22 March 2001

Ten chlorate-respiring bacteria were isolated from wastewater and a perchlorate-degrading bioreactor. Eight of the isolates were able to degrade perchlorate, and all isolates used oxygen and chlorate as terminal electron acceptors. The growth kinetics of two perchlorate-degrading isolates, designated "Dechlorosoma" sp. strains KJ and PDX, were examined with acetate as the electron donor in batch tests. The maximum observed aerobic growth rates of KJ and PDX (0.27 and 0.28 h-1, respectively) were only slightly higher than the anoxic growth rates obtained by these isolates during growth with chlorate (0.26 and 0.21 h-1, respectively). The maximum observed growth rates of the two non-perchlorate-utilizing isolates (PDA and PDB) were much higher under aerobic conditions (0.64 and 0.41 h-1, respectively) than under anoxic (chlorate-reducing) conditions (0.18 and 0.21 h-1, respectively). The maximum growth rates of PDX on perchlorate and chlorate were identical (0.21 h-1) and exceeded that of strain KJ on perchlorate (0.14 h-1). Growth of one isolate (PDX) was more rapid on acetate than on lactate. There were substantial differences in the half-saturation constants measured for anoxic growth of isolates on acetate with excess perchlorate (470 mg/liter for KJ and 45 mg/liter for PDX). Biomass yields (grams of cells per gram of acetate) for strain KJ were not statistically different in the presence of the electron acceptors oxygen (0.46 ± 0.07 [n = 7]), chlorate (0.44 ± 0.05 [n = 7]), and perchlorate (0.50 ± 0.08 [n = 7]). These studies provide evidence that facultative microorganisms with the capability for perchlorate and chlorate respiration exist, that not all chlorate-respiring microorganisms are capable of anoxic growth on perchlorate, and that isolates have dissimilar growth kinetics using different electron donors and acceptors.

* Corresponding author. Mailing address: Dept. of Civil and Environmental Engineering, 212 Sackett Bldg., The Pennsylvania State University, University Park, PA 16802. Phone: (814) 863-7908. Fax: (814) 863-7304. E-mail: blogan{at}psu.edu.

Applied and Environmental Microbiology, June 2001, p. 2499-2506, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2499-2506.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nozawa-Inoue, M., Jien, M., Hamilton, N. S., Stewart, V., Scow, K. M., Hristova, K. R. (2008). Quantitative Detection of Perchlorate-Reducing Bacteria by Real-Time PCR Targeting the Perchlorate Reductase Gene. Appl. Environ. Microbiol. 74: 1941-1944 [Abstract] [Full Text]  
  • Nozawa-Inoue, M., Scow, K. M., Rolston, D. E. (2005). Reduction of Perchlorate and Nitrate by Microbial Communities in Vadose Soil. Appl. Environ. Microbiol. 71: 3928-3934 [Abstract] [Full Text]  
  • Bender, K. S., Rice, M. R., Fugate, W. H., Coates, J. D., Achenbach, L. A. (2004). Metabolic Primers for Detection of (Per)chlorate-Reducing Bacteria in the Environment and Phylogenetic Analysis of cld Gene Sequences. Appl. Environ. Microbiol. 70: 5651-5658 [Abstract] [Full Text]  
  • Bender, K. S., O'Connor, S. M., Chakraborty, R., Coates, J. D., Achenbach, L. A. (2002). Sequencing and Transcriptional Analysis of the Chlorite Dismutase Gene of Dechloromonas agitata and Its Use as a Metabolic Probe. Appl. Environ. Microbiol. 68: 4820-4826 [Abstract] [Full Text]  
  • Chaudhuri, S. K., O'Connor, S. M., Gustavson, R. L., Achenbach, L. A., Coates, J. D. (2002). Environmental Factors That Control Microbial Perchlorate Reduction. Appl. Environ. Microbiol. 68: 4425-4430 [Abstract] [Full Text]  
  • O'Connor, S. M., Coates, J. D. (2002). Universal Immunoprobe for (Per)Chlorate-Reducing Bacteria. Appl. Environ. Microbiol. 68: 3108-3113 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»