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Applied and Environmental Microbiology, June 2001, p. 2526-2530, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2526-2530.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Synthesis and Fermentation Properties of Novel Galacto-Oligosaccharides by beta -Galactosidases from Bifidobacterium Species

Bodun A. Rabiu,1 Andrew J. Jay,2 Glenn R. Gibson,1 and Robert A. Rastall1,*

Division of Food Microbial Sciences, School of Food Biosciences, The University of Reading, Whiteknights, Reading RG6 6AP,1 and Institute of Food Research, Colney Lane, Norwich NR4 7UA,2 United Kingdom

Received 11 November 2000/Accepted 20 March 2001

beta -Galactosidase enzymes were extracted from pure cultures of Bifidobacterium angulatum, B. bifidum BB-12, B. adolescentis ANB-7, B. infantis DSM-20088, and B. pseudolongum DSM-20099 and used in glycosyl transfer reactions to synthesize oligosaccharides from lactose. At a lactose concentration of 30% (wt/wt) oligosaccharide yields of 24.7 to 47.6% occurred within 7 h. Examination of the products by thin-layer chromatography and methylation analysis revealed distinct product derived spectra from each enzyme. These were found to be different to that of Oligomate 55, a commercial prebiotic galacto-oligosaccharide. Fermentation testing of the oligosaccharides showed an increase in growth rate, compared to Oligomate 55, with products derived from B. angulatum, B. bifidum, B. infantis, and B. pseudolongum. However B. adolescentis had a lower growth rates on its oligosaccharide compared with Oligomate 55. Mixed culture testing of the B. bifidum BS-4 oligosaccharide showed that the overall prebiotic effect was equivalent to that of Oligomate 55.

* Corresponding author. Mailing address: School of Food Biosciences, The University of Reading, P.O. Box 226, Whiteknights, Reading RG6 6AP, United Kingdom. Phone: 118-9316726, Fax: 118-9310080. E-mail: R.A.Rastall{at}afnovell.reading.ac.uk.

Applied and Environmental Microbiology, June 2001, p. 2526-2530, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2526-2530.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.