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Applied and Environmental Microbiology, June 2001, p. 2692-2698, Vol. 67, No. 6
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.6.2692-2698.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Analysis of the Role of OpuC, an Osmolyte Transport System, in
Salt Tolerance and Virulence Potential of Listeria
monocytogenes
Roy D.
Sleator,1
Jeroen
Wouters,2
Cormac G. M.
Gahan,1
Tjakko
Abee,2 and
Colin
Hill1,*
Department of Microbiology and National Food
Biotechnology Centre, University College, Cork,
Ireland,1 and Laboratory of Food
Microbiology, Wageningen University, and Wageningen Centre for Food
Sciences, Wageningen, The Netherlands2
Received 16 October 2000/Accepted 18 March 2001
The success of Listeria monocytogenes as a food-borne
pathogen owes much to its ability to survive a variety of stresses, both in the external environment prior to ingestion and subsequently within the animal host. Growth at high salt concentrations and low
temperatures is attributed mainly to the accumulation of organic solutes such as glycine betaine and carnitine. We utilized a novel system for generating chromosomal mutations (based on a lactococcal pWVO1-derived Ori+ RepA
vector, pORI19) to
identify a listerial OpuC homologue. Mutating the operon
in two strains of L. monocytogenes revealed significant strain variation in the observed activity of OpuC. Radiolabeled osmolyte uptake studies, together with growth experiments in defined media, linked OpuC to carnitine and glycine betaine uptake in Listeria. We also investigated the role of OpuC in
contributing to the growth and survival of Listeria in an
animal (murine) model of infection. Altering OpuC resulted in a
significant reduction in the ability of Listeria to
colonize the upper small intestine and cause subsequent systemic
infection following peroral inoculation.
*
Corresponding author. Mailing address: Department of
Microbiology, University College, Cork, Ireland. Phone: 353-21-902397. Fax: 353-21-903101. E-mail: c.hill{at}ucc.ie.
Applied and Environmental Microbiology, June 2001, p. 2692-2698, Vol. 67, No. 6
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.6.2692-2698.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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