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Applied and Environmental Microbiology, June 2001, p. 2829-2832, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2829-2832.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Biotransformation of D-Limonene to (+) trans-Carveol by Toluene-Grown Rhodococcus opacus PWD4 Cells

Wouter A. Duetz,1,* Ann H. M. Fjällman,1 Shuyu Ren,1 Catherine Jourdat,2 and Bernard Witholt1

Institute of Biotechnology, ETH Hönggerberg, HPT, CH 8093, Zürich, Switzerland,1 and Rhône Poulenc-CRIT, 69153 Décines Charpieu cedex, France2

Received 19 January 2001/Accepted 12 March 2001

The toluene-degrading strain Rhodococcus opacus PWD4 was found to hydroxylate D-limonene exclusively in the 6-position, yielding enantiomerically pure (+) trans-carveol and traces of (+) carvone. This biotransformation was studied using cells cultivated in chemostat culture with toluene as a carbon and energy source. The maximal specific activity of (+) trans-carveol formation was 14.7 U (g of cells [dry weight])-1, and the final yield was 94 to 97%. Toluene was found to be a strong competitive inhibitor of the D-limonene conversion. Glucose-grown cells did not form any trans-carveol from D-limonene. These results suggest that one of the enzymes involved in toluene degradation is responsible for this allylic monohydroxylation. Another toluene degrader (Rhodococcus globerulus PWD8) had a lower specific activity but was found to oxidize most of the formed trans-carveol to (+) carvone, allowing for the biocatalytic production of this flavor compound.

* Corresponding author. Mailing address: Institute of Biotechnology, ETH Hönggerberg, HPT, CH 8093, Zürich, Switzerland. Phone: (41)-1-6333811. Fax: (41)-1-6331051. E-mail: duetz{at}biotech.biol.ethz.ch.

Applied and Environmental Microbiology, June 2001, p. 2829-2832, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2829-2832.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • van Beilen, J. B., Holtackers, R., Luscher, D., Bauer, U., Witholt, B., Duetz, W. A. (2005). Biocatalytic Production of Perillyl Alcohol from Limonene by Using a Novel Mycobacterium sp. Cytochrome P450 Alkane Hydroxylase Expressed in Pseudomonas putida. Appl. Environ. Microbiol. 71: 1737-1744 [Abstract] [Full Text]  
  • Spain, J. C., Nishino, S. F., Witholt, B., Tan, L.-S., Duetz, W. A. (2003). Production of 6-Phenylacetylene Picolinic Acid from Diphenylacetylene by a Toluene-Degrading Acinetobacter Strain. Appl. Environ. Microbiol. 69: 4037-4042 [Abstract] [Full Text]  


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