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Applied and Environmental Microbiology, June 2001, p. 2833-2836, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2833-2836.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Inactivation of Mycobacterium paratuberculosis by Pulsed Electric Fields

Neil J. Rowan,1,* Scott J. MacGregor,2 John G. Anderson,1 Douglas Cameron,1 and Owen Farish2

Department of Bioscience and Biotechnology1 and Department of Electronic and Electrical Engineering,2 University of Strathclyde, Glasgow, Scotland

Received 21 December 2000/Accepted 19 March 2001

The influence of treatment temperature and pulsed electric fields (PEF) on the viability of Mycobacterium paratuberculosis cells suspended in 0.1% (wt/vol) peptone water and in sterilized cow's milk was assessed by direct viable counts and by transmission electron microscopy (TEM). PEF treatment at 50°C (2,500 pulses at 30 kV/cm) reduced the level of viable M. paratuberculosis cells by approximately 5.3 and 5.9 log10 CFU/ml in 0.1% peptone water and in cow's milk, respectively, while PEF treatment of M. paratuberculosis at lower temperatures resulted in less lethality. Heating alone at 50°C for 25 min or at 72°C for 25 s (extended high-temperature, short-time pasteurization) resulted in reductions of M. paratuberculosis of approximately 0.01 and 2.4 log10 CFU/ml, respectively. TEM studies revealed that exposure to PEF treatment resulted in substantial damage at the cellular level to M. paratuberculosis.

* Corresponding author. Mailing address: Department of Bioscience and Biotechnology, University of Strathclyde, Royal College, 204 George St., Glasgow G1 1XW, Scotland. Phone: 44 (0)141 548 2531. Fax: 44 (0)141 553 4124. E-mail: n.j.rowan{at}strath.ac.uk.

Applied and Environmental Microbiology, June 2001, p. 2833-2836, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2833-2836.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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