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Applied and Environmental Microbiology, June 2001, p. 2849-2852, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2849-2852.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Quantitative PCR Method for Diagnosis of Citrus Bacterial Canker†

J. Cubero,1 J. H. Graham,1,* and T. R. Gottwald2

University of Florida, Citrus Research and Education Center, Lake Alfred, Florida 33850,1 and USDA-ARS-USHRL, Ft. Pierce, Florida, 349452

Received 4 December 2000/Accepted 28 March 2001

For diagnosis of citrus bacterial canker by PCR, an internal standard is employed to ensure the quality of the DNA extraction and that proper requisites exist for the amplification reaction. The ratio of PCR products from the internal standard and bacterial target is used to estimate the initial bacterial concentration in citrus tissues with lesions.

* Corresponding author. Mailing address: James H. Graham, University of Florida, Citrus Research and Education Center (CREC), 700 Experiment Station Rd., Lake Alfred, FL 33850-2299. Phone: (863) 956-1151. Fax: (863) 956-4631. E-mail: jhg{at}LAL.UFL.EDU.

dagger Florida Agricultural Experiment Station Journal Series no. R-07912.

Applied and Environmental Microbiology, June 2001, p. 2849-2852, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2849-2852.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.