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Applied and Environmental Microbiology, July 2001, p. 2895-2902, Vol. 67, No. 7
Department of Food Science and Technology,
Mississippi Agricultural and Forestry Experiment Station, Mississippi
State University, Mississippi State, Mississippi
397621 and Gulf Coast Seafood
Laboratory, U.S. Food and Drug Administration, Dauphin Island,
Alabama 365282
Received 8 January 2001/Accepted 15 April 2001
Viable counts of three strains of Vibrio vulnificus and
its phage were determined during exposure to a mechanical
gastrointestinal model with or without antacid for 9 h at 37°C.
V. vulnificus was eliminated (>4-log reduction) within 30 min in the gastric compartment (pH decline from 5.0 to 3.5). Viable
V. vulnificus cells delivered from the gastric compartment
during the first 30 min of exposure reached 106 to
108 CFU/ml in the intestinal compartment after 9 h (pH
7.0). Phages were eliminated within 45 min in the gastric compartment
(pH decline from 5.1 to 2.5). Less than a 2-log reduction of phage was
observed in the intestinal compartment after 9 h (pH 7.0).
When the gastric compartment contained antacid V. vulnificus counts decreased slightly (<2 log) during 2 h of
exposure (pH decline from 7.7 to 6.0), while counts in the intestinal
compartment (pH 7.5) reached 107 to 109 CFU/ml.
Phage numbers decreased 1 log after 2 h in the gastric compartment
(pH decline from 7.7 to 5.7) containing antacid and decreased 1 log in
the intestinal compartment (pH 7.6) after 9 h. Presence of antacid
in the gastric compartment of the model greatly increased the ability
of both V. vulnificus and its phage to survive simulated
gastrointestinal transit and may be a factor involved with
oyster-associated illness.
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.7.2895-2902.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Antacid Increases Survival of Vibrio
vulnificus and Vibrio vulnificus Phage in a
Gastrointestinal Model
*
Corresponding author. Mailing address: Department of
Food Science and Technology, Mississippi Agricultural and Forestry
Experiment Station, Mississippi State University, Box 9805, Mississippi
State, MS 39762-9805. Phone: (662) 325-8722. Fax: (662) 325-8728. E-mail: microman{at}ra.msstate.edu.
Approved for publication as journal article no. J9772 of the
Mississippi Agricultural and Forestry Experiment Station.
Present address: Virginia Seafood Agricultural Research and
Extension Center, Hampton, VA 23669.
Applied and Environmental Microbiology, July 2001, p. 2895-2902, Vol. 67, No. 7
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.7.2895-2902.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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