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Applied and Environmental Microbiology, July 2001, p. 2903-2907, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.2903-2907.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The Functional ccpA Gene Is Required for Carbon Catabolite Repression in Lactobacillus plantarum

Lidia Muscariello,1 Rosangela Marasco,2 Maurilio De Felice,3 and Margherita Sacco1,4,*

Istituto Internazionale di Genetica e Biofisica, Consiglio Nazionale delle Ricerche, 80125 Naples,1 Facoltà di Scienze MM FF NN, Università degli Studi del Sannio, 82100 Benevento,2 Sezione di Microbiologia, Dipartimento di Fisiologia Generale ed Ambientale, Università, Federico II, 80134 Naples,3 and Dipartimento di Scienze Ambientali, Seconda Università di Napoli, 81100 Caserta,4 Italy

Received 27 November 2000/Accepted 16 April 2001

We report the characterization of the ccpA gene of Lactobacillus plantarum, coding for catabolite control protein A. The gene is linked to the pepQ gene, encoding a proline peptidase, in the order ccpA-pepQ, with the two genes transcribed in tandem from the same strand as distinct transcriptional units. Two ccpA transcription start sites corresponding to two functional promoters were found, expression from the upstream promoter being autogenously regulated through a catabolite-responsive element (cre) sequence overlapping the upstream +1 site. During growth on ribose, the upstream promoter showed maximal expression, while growth on glucose led to transcription from the downstream promoter. In a ccpA mutant strain, the gene was transcribed mainly from the upstream promoter in both repressing and non repressing conditions. Expression of two enzyme activities, beta -glucosidase and beta -galactosidase, was relieved from carbon catabolite repression in the ccpA mutant strain. In vivo footprinting analysis of the catabolite-controlled bglH gene regulatory region in the ccpA mutant strain showed loss of protection of the cre under repressing conditions.

* Corresponding author. Mailing address: Istituto Internazionale di Genetica e Biofisica, Consiglio Nazionale delle Ricerche, via G. Marconi 10, 80125 Naples, Italy. Phone: 39 081 7257219. Fax: 39 081 5936123. E-mail: sacco{at}iigbna.iigb.na.cnr.it.

Applied and Environmental Microbiology, July 2001, p. 2903-2907, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.2903-2907.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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