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Applied and Environmental Microbiology, July 2001, p. 3029-3032, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3029-3032.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Low-Pressure UV Inactivation and DNA Repair Potential of Cryptosporidium parvum Oocysts

Gwy-Am Shin,1,* Karl G. Linden,2 Michael J. Arrowood,3 and Mark D. Sobsey1

Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-74001; Department of Civil & Environmental Engineering, Duke University, Durham, North Carolina 277082; and Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303033

Received 7 December 2000/Accepted 22 April 2001

Because Cryptosporidium parvum oocysts are very resistant to conventional water treatment processes, including chemical disinfection, we determined the kinetics and extent of their inactivation by monochromatic, low-pressure (LP), mercury vapor lamp UV radiation and their subsequent potential for DNA repair of UV damage. A UV collimated-beam apparatus was used to expose suspensions of purified C. parvum oocysts in phosphate-buffered saline, pH 7.3, at 25°C to various doses of monochromatic LP UV. C. parvum infectivity reductions were rapid, approximately first order, and at a dose of 3 mJ/cm2 (=30 J/m2), the reduction reached the cell culture assay detection limit of ~3 log10. At UV doses of 1.2 and 3 mJ/cm2, the log10 reductions of C. parvum oocyst infectivity were not significantly different for control oocysts and those exposed to dark or light repair conditions for UV-induced DNA damage. These results indicate that C. parvum oocysts are very sensitive to inactivation by low doses of monochromatic LP UV radiation and that there is no phenotypic evidence of either light or dark repair of UV-induced DNA damage.

* Corresponding author. Mailing address: Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7400. Phone: (919) 966-7316. Fax: (919) 966-4711. E-mail: gwyam{at}isis.unc.edu.

Applied and Environmental Microbiology, July 2001, p. 3029-3032, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3029-3032.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.