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Applied and Environmental Microbiology, July 2001, p. 3041-3045, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3041-3045.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of a Heme-Dependent Catalase from Methanobrevibacter arboriphilus†

Seigo Shima,1,* Melanie Sordel-Klippert,1 Andrei Brioukhanov,2 Alexander Netrusov,2 Dietmar Linder,3 and Rudolf K. Thauer1

Max-Planck-Institut für Terrestrische Mikrobiologie and Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps-Universität, D-35043 Marburg,1 and Biochemisches Institut, Fachbereich Humanmedizin, Justus-Liebig-Universität, D-35392 Gießen,3 Germany, and Microbiology Department, Moscow Lomonosov State University, Moscow 119899, Russia2

Received 2 February 2001/Accepted 3 May 2001

Recently it was reported that methanogens of the genus Methanobrevibacter exhibit catalase activity. This was surprising, since Methanobrevibacter species belong to the order Methanobacteriales, which are known not to contain cytochromes and to lack the ability to synthesize heme. We report here that Methanobrevibacter arboriphilus strains AZ and DH1 contained catalase activity only when the growth medium was supplemented with hemin. The heme catalase was purified and characterized, and the encoding gene was cloned. The amino acid sequence of the catalase from the methanogens is most similar to that of Methanosarcina barkeri.

* Corresponding author. Mailing address: Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Strasse, D-35043 Marburg, Germany. Phone: (49) 6421-178200. Fax: (49) 6421-178209. E-mail: shima{at}mailer.uni-marburg.de.

dagger Dedicated to Hans Trüper on the occasion of his 65th birthday.

Applied and Environmental Microbiology, July 2001, p. 3041-3045, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3041-3045.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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