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Applied and Environmental Microbiology, July 2001, p. 3161-3167, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3161-3167.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Duplication of Hemolysin Genes in a Virulent Isolate of Vibrio harveyi

X.-H. Zhang, P. G. Meaden, and B. Austin*

Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland

Received 22 January 2001/Accepted 27 April 2001

Vibrio harveyi VIB 645, which is very pathogenic towards salmonids and produces extracellular product with a high titer of hemolytic activity towards fish erythrocytes, was found to contain two closely related hemolysin genes (designated vhhA and vhhB), whereas the majority of strains examined (11 of 13) carried only a single hemolysin gene. Both genes from VIB 645 were cloned and sequenced. The open reading frames (ORFs) of vhhA and vhhB shared a high level of identity (98.8%) and were predicted to encode identical polypeptides comprising 418 amino acid residues. The VHH protein shows homology to the lecithinase of V. mimicus and V. cholerae. Transformants of Escherichia coli containing the ORF of either vhhA or vhhB displayed weak hemolytic activity in rainbow trout blood agar. The hemolytic activity was very high when the ORF of vhhB was cloned in E. coli together with the native promoter. Surprisingly, the level of vhh-specific RNA transcript produced by VIB 645 was found to be very low. We conclude that the hemolytic phenotype of VIB 645 is not due to increased expression of one or both copies of the vhh gene.

* Corresponding author. Mailing address: Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, United Kingdom. Phone: 44-131-451-3452. Fax: 44-131-451-3009. E-mail: b.austin{at}hw.ac.uk.

Applied and Environmental Microbiology, July 2001, p. 3161-3167, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3161-3167.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sun, B., Zhang, X.-H., Tang, X., Wang, S., Zhong, Y., Chen, J., Austin, B. (2007). A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot (Scophthalmus maximus). J. Bacteriol. 189: 2575-2579 [Abstract] [Full Text]  
  • Zhong, Y., Zhang, X.-H., Chen, J., Chi, Z., Sun, B., Li, Y., Austin, B. (2006). Overexpression, Purification, Characterization, and Pathogenicity of Vibrio harveyi Hemolysin VHH.. Infect. Immun. 74: 6001-6005 [Abstract] [Full Text]  
  • Henke, J. M., Bassler, B. L. (2004). Quorum Sensing Regulates Type III Secretion in Vibrio harveyi and Vibrio parahaemolyticus. J. Bacteriol. 186: 3794-3805 [Abstract] [Full Text]  


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