This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chizhikov, V.
Right arrow Articles by Levy, D. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chizhikov, V.
Right arrow Articles by Levy, D. D.
Agricola
Right arrow Articles by Chizhikov, V.
Right arrow Articles by Levy, D. D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2001, p. 3258-3263, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3258-3263.2001

Microarray Analysis of Microbial Virulence Factors

Vladimir Chizhikov,1 Avraham Rasooly,2,* Konstantin Chumakov,1 and Dan D. Levy2

Food and Drug Administration Center for Biologics Evaluation and Research, Rockville, Maryland,1 and Food and Drug Administration Center for Food Safety and Applied Nutrition, Washington, D.C.2

Received 1 December 2000/Accepted 22 March 2001

Hybridization with oligonucleotide microchips (microarrays) was used for discrimination among strains of Escherichia coli and other pathogenic enteric bacteria harboring various virulence factors. Oligonucleotide microchips are miniature arrays of gene-specific oligonucleotide probes immobilized on a glass surface. The combination of this technique with the amplification of genetic material by PCR is a powerful tool for the detection of and simultaneous discrimination among food-borne human pathogens. The presence of six genes (eaeA, slt-I, slt-II, fliC, rfbE, and ipaH) encoding bacterial antigenic determinants and virulence factors of bacterial strains was monitored by multiplex PCR followed by hybridization of the denatured PCR product to the gene-specific oligonucleotides on the microchip. The assay was able to detect these virulence factors in 15 Salmonella, Shigella, and E. coli strains. The results of the chip analysis were confirmed by hybridization of radiolabeled gene-specific probes to genomic DNA from bacterial colonies. In contrast, gel electrophoretic analysis of the multiplex PCR products used for the microarray analysis produced ambiguous results due to the presence of unexpected and uncharacterized bands. Our results suggest that microarray analysis of microbial virulence factors might be very useful for automated identification and characterization of bacterial pathogens.

* Corresponding author. Mailing address: Food and Drug Administration, 200 C St. SW, Washington, DC 20204. Phone: (202) 205-4192. Fax: (202) 401-7740. E-mail: axr{at}vm.cfsan.fda.gov.

Applied and Environmental Microbiology, July 2001, p. 3258-3263, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3258-3263.2001


This article has been cited by other articles:

  • Miller, M. B., Tang, Y.-W. (2009). Basic Concepts of Microarrays and Potential Applications in Clinical Microbiology. Clin. Microbiol. Rev. 22: 611-633 [Abstract] [Full Text]  
  • Miller, S. M., Tourlousse, D. M., Stedtfeld, R. D., Baushke, S. W., Herzog, A. B., Wick, L. M., Rouillard, J. M., Gulari, E., Tiedje, J. M., Hashsham, S. A. (2008). In Situ-Synthesized Virulence and Marker Gene Biochip for Detection of Bacterial Pathogens in Water. Appl. Environ. Microbiol. 74: 2200-2209 [Abstract] [Full Text]  
  • Anjum, M. F., Mafura, M., Slickers, P., Ballmer, K., Kuhnert, P., Woodward, M. J., Ehricht, R. (2007). Pathotyping Escherichia coli by Using Miniaturized DNA Microarrays. Appl. Environ. Microbiol. 73: 5692-5697 [Abstract] [Full Text]  
  • Han, W., Liu, B., Cao, B., Beutin, L., Kruger, U., Liu, H., Li, Y., Liu, Y., Feng, L., Wang, L. (2007). DNA Microarray-Based Identification of Serogroups and Virulence Gene Patterns of Escherichia coli Isolates Associated with Porcine Postweaning Diarrhea and Edema Disease. Appl. Environ. Microbiol. 73: 4082-4088 [Abstract] [Full Text]  
  • Lin, B., Blaney, K. M., Malanoski, A. P., Ligler, A. G., Schnur, J. M., Metzgar, D., Russell, K. L., Stenger, D. A. (2007). Using a Resequencing Microarray as a Multiple Respiratory Pathogen Detection Assay. J. Clin. Microbiol. 45: 443-452 [Abstract] [Full Text]  
  • Neverov, A. A., Riddell, M. A., Moss, W. J., Volokhov, D. V., Rota, P. A., Lowe, L. E., Chibo, D., Smit, S. B., Griffin, D. E., Chumakov, K. M., Chizhikov, V. E. (2006). Genotyping of measles virus in clinical specimens on the basis of oligonucleotide microarray hybridization patterns.. J. Clin. Microbiol. 44: 3752-3759 [Abstract] [Full Text]  
  • Cleven, B. E. E., Palka-Santini, M., Gielen, J., Meembor, S., Kronke, M., Krut, O. (2006). Identification and characterization of bacterial pathogens causing bloodstream infections by DNA microarray.. J. Clin. Microbiol. 44: 2389-2397 [Abstract] [Full Text]  
  • Bruant, G., Maynard, C., Bekal, S., Gaucher, I., Masson, L., Brousseau, R., Harel, J. (2006). Development and Validation of an Oligonucleotide Microarray for Detection of Multiple Virulence and Antimicrobial Resistance Genes in Escherichia coli.. Appl. Environ. Microbiol. 72: 3780-3784 [Abstract] [Full Text]  
  • Garrido, P., Blanco, M., Moreno-Paz, M., Briones, C., Dahbi, G., Blanco, J., Blanco, J., Parro, V. (2006). STEC-EPEC Oligonucleotide Microarray: A New Tool for Typing Genetic Variants of the LEE Pathogenicity Island of Human and Animal Shiga Toxin-Producing Escherichia coli (STEC) and Enteropathogenic E. coli (EPEC) Strains. Clin. Chem. 52: 192-201 [Abstract] [Full Text]  
  • Denkin, S., Volokhov, D., Chizhikov, V., Zhang, Y. (2005). Microarray-based pncA genotyping of pyrazinamide-resistant strains of Mycobacterium tuberculosis. J Med Microbiol 54: 1127-1131 [Abstract] [Full Text]  
  • Peytavi, R., Raymond, F. R., Gagne, D., Picard, F. J., Jia, G., Zoval, J., Madou, M., Boissinot, K., Boissinot, M., Bissonnette, L., Ouellette, M., Bergeron, M. G. (2005). Microfluidic Device for Rapid (<15 min) Automated Microarray Hybridization. Clin. Chem. 51: 1836-1844 [Abstract] [Full Text]  
  • Letowski, J., Bravo, A., Brousseau, R., Masson, L. (2005). Assessment of cry1 Gene Contents of Bacillus thuringiensis Strains by Use of DNA Microarrays. Appl. Environ. Microbiol. 71: 5391-5398 [Abstract] [Full Text]  
  • Wang, Z., Orlandi, P. A., Stenger, D. A. (2005). Simultaneous Detection of Four Human Pathogenic Microsporidian Species from Clinical Samples by Oligonucleotide Microarray. J. Clin. Microbiol. 43: 4121-4128 [Abstract] [Full Text]  
  • Park, H., Jang, H., Song, E., Chang, C. L., Lee, M., Jeong, S., Park, J., Kang, B., Kim, C. (2005). Detection and Genotyping of Mycobacterium Species from Clinical Isolates and Specimens by Oligonucleotide Array. J. Clin. Microbiol. 43: 1782-1788 [Abstract] [Full Text]  
  • Panicker, G., Call, D. R., Krug, M. J., Bej, A. K. (2004). Detection of Pathogenic Vibrio spp. in Shellfish by Using Multiplex PCR and DNA Microarrays. Appl. Environ. Microbiol. 70: 7436-7444 [Abstract] [Full Text]  
  • Ivshina, A. V., Vodeiko, G. M., Kuznetsov, V. A., Volokhov, D., Taffs, R., Chizhikov, V. I., Levandowski, R. A., Chumakov, K. M. (2004). Mapping of Genomic Segments of Influenza B Virus Strains by an Oligonucleotide Microarray Method. J. Clin. Microbiol. 42: 5793-5801 [Abstract] [Full Text]  
  • Boriskin, Y. S., Rice, P. S., Stabler, R. A., Hinds, J., Al-Ghusein, H., Vass, K., Butcher, P. D. (2004). DNA Microarrays for Virus Detection in Cases of Central Nervous System Infection. J. Clin. Microbiol. 42: 5811-5818 [Abstract] [Full Text]  
  • Jang, H., Cho, M., Heo, J., Kim, H., Jun, H., Shin, W., Cho, B., Park, H., Kim, C. (2004). Oligonucleotide Chip for Detection of Lamivudine-Resistant Hepatitis B Virus. J. Clin. Microbiol. 42: 4181-4188 [Abstract] [Full Text]  
  • Maekawa, M., Nagaoka, T., Taniguchi, T., Higashi, H., Sugimura, H., Sugano, K., Yonekawa, H., Satoh, T., Horii, T., Shirai, N., Takeshita, A., Kanno, T. (2004). Three-Dimensional Microarray Compared with PCR-Single-Strand Conformation Polymorphism Analysis/DNA Sequencing for Mutation Analysis of K-ras Codons 12 and 13. Clin. Chem. 50: 1322-1327 [Abstract] [Full Text]  
  • Warsen, A. E., Krug, M. J., LaFrentz, S., Stanek, D. R., Loge, F. J., Call, D. R. (2004). Simultaneous Discrimination between 15 Fish Pathogens by Using 16S Ribosomal DNA PCR and DNA Microarrays. Appl. Environ. Microbiol. 70: 4216-4221 [Abstract] [Full Text]  
  • Wang, Z., Vora, G. J., Stenger, D. A. (2004). Detection and Genotyping of Entamoeba histolytica, Entamoeba dispar, Giardia lamblia, and Cryptosporidium parvum by Oligonucleotide Microarray. J. Clin. Microbiol. 42: 3262-3271 [Abstract] [Full Text]  
  • Vora, G. J., Meador, C. E., Stenger, D. A., Andreadis, J. D. (2004). Nucleic Acid Amplification Strategies for DNA Microarray-Based Pathogen Detection. Appl. Environ. Microbiol. 70: 3047-3054 [Abstract] [Full Text]  
  • Gonzalez, S. F., Krug, M. J., Nielsen, M. E., Santos, Y., Call, D. R. (2004). Simultaneous Detection of Marine Fish Pathogens by Using Multiplex PCR and a DNA Microarray. J. Clin. Microbiol. 42: 1414-1419 [Abstract] [Full Text]  
  • Volokhov, D., Chizhikov, V., Chumakov, K., Rasooly, A. (2003). Microarray-Based Identification of Thermophilic Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis. J. Clin. Microbiol. 41: 4071-4080 [Abstract] [Full Text]  
  • Strommenger, B., Kettlitz, C., Werner, G., Witte, W. (2003). Multiplex PCR Assay for Simultaneous Detection of Nine Clinically Relevant Antibiotic Resistance Genes in Staphylococcus aureus. J. Clin. Microbiol. 41: 4089-4094 [Abstract] [Full Text]  
  • Cherkasova, E., Laassri, M., Chizhikov, V., Korotkova, E., Dragunsky, E., Agol, V. I., Chumakov, K. (2003). Microarray analysis of evolution of RNA viruses: Evidence of circulation of virulent highly divergent vaccine-derived polioviruses. Proc. Natl. Acad. Sci. USA 100: 9398-9403 [Abstract] [Full Text]  
  • Bekal, S., Brousseau, R., Masson, L., Prefontaine, G., Fairbrother, J., Harel, J. (2003). Rapid Identification of Escherichia coli Pathotypes by Virulence Gene Detection with DNA Microarrays. J. Clin. Microbiol. 41: 2113-2125 [Abstract] [Full Text]  
  • Dobrindt, U., Agerer, F., Michaelis, K., Janka, A., Buchrieser, C., Samuelson, M., Svanborg, C., Gottschalk, G., Karch, H., Hacker, J. (2003). Analysis of Genome Plasticity in Pathogenic and Commensal Escherichia coli Isolates by Use of DNA Arrays. J. Bacteriol. 185: 1831-1840 [Abstract] [Full Text]  
  • Volokhov, D., Rasooly, A., Chumakov, K., Chizhikov, V. (2002). Identification of Listeria Species by Microarray-Based Assay. J. Clin. Microbiol. 40: 4720-4728 [Abstract] [Full Text]  
  • Chizhikov, V., Wagner, M., Ivshina, A., Hoshino, Y., Kapikian, A. Z., Chumakov, K. (2002). Detection and Genotyping of Human Group A Rotaviruses by Oligonucleotide Microarray Hybridization. J. Clin. Microbiol. 40: 2398-2407 [Abstract] [Full Text]  
  • Garaizar, J., Porwollik, S., Echeita, A., Rementeria, A., Herrera, S., Wong, R. M.-Y., Frye, J., Usera, M. A., McClelland, M. (2002). DNA Microarray-Based Typing of an Atypical Monophasic Salmonella enterica Serovar. J. Clin. Microbiol. 40: 2074-2078 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»