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Applied and Environmental Microbiology, July 2001, p. 3291-3294, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3291-3294.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Development of a Quantitative Competitive PCR Assay for Detection and Quantification of Escherichia coli O157:H7 Cells

Wenli Li and Mary Anne Drake*

Department of Food Science and Technology, Mississippi State University, Mississippi State, Mississippi 39762-9805

Received 13 October 2000/Accepted 2 May 2001

A quantitative competitive PCR (QC-PCR) assay was developed to detect and quantify Escherichia coli O157:H7 cells. From 103 to 108 CFU of E. coli O157:H7 cells/ml was quantified in broth or skim milk, and cell densities predicted by QC-PCR were highly related to viable cell counts (r2 = 0.99 and 0.93, respectively). QC-PCR has potential for quantitative detection of pathogenic bacteria in foods.

* Corresponding author. Current address: Department of Food Science, Southeast Dairy Foods Research Center, Raleigh, NC 27695-7624. Phone: (919) 513-4598. Fax: (919) 515-7124. E-mail: mdrake{at}unity.ncsu.edu.

Applied and Environmental Microbiology, July 2001, p. 3291-3294, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3291-3294.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.