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Applied and Environmental Microbiology, July 2001, p. 3295-3298, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3295-3298.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Approach To Determine rrn Arrangement in Salmonella Serovars

R. Allen Helm1 and Stanley Maloy1,2,*

Department of Microbiology, University of Illinois, Urbana, Illinois,1 and Center for Microbial Sciences, San Diego, California2

Received 12 March 2001/Accepted 30 April 2001

A PCR method was developed by which to rapidly and accurately determine the rrn arrangement of Salmonella enterica serovars. Primers were designed to the genomic regions flanking each of the seven rrn operons. PCR analysis using combinations of these primers will distinguish each of the possible arrangements of the rrn skeleton.

* Corresponding author. Mailing address: Department of Microbiology, University of Illinois, CLSL B103, 601 S. Goodwin Ave., Urbana, IL 61801. Phone: (217) 333-3122. Fax: (217) 244-6697. E-mail: s-maloy{at}life.uiuc.edu.

Applied and Environmental Microbiology, July 2001, p. 3295-3298, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3295-3298.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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