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Applied and Environmental Microbiology, July 2001, p. 3304-3308, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3304-3308.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Organization and Regulation of meta Cleavage Pathway Genes for Toluene and o-Xylene Derivative Degradation in Pseudomonas stutzeri OX1

Fabio L. G. Arenghi,1 Davide Berlanda,1 Enrica Galli,1 Guido Sello,2 and Paola Barbieri1,3,*

Dipartimento di Genetica e di Biologia dei Microrganismi1 and Dipartimento di Chimica Organica e Industriale,2 Università degli Studi di Milano, Milano, and Dipartimento di Biologia Strutturale e Funzionale, Università dell'Insubria, Varese,3 Italy

Received 29 January 2001/Accepted 2 May 2001

Pseudomonas stutzeri OX1 meta pathway genes for toluene and o-xylene catabolism were analyzed, and loci encoding phenol hydroxylase, catechol 2,3-dioxygenase, 2-hydroxymuconate semialdehyde dehydrogenase, and 2-hydroxymuconate semialdehyde hydrolase were mapped. Phenol hydroxylase converted a broad range of substrates, as it was also able to transform the nongrowth substrates 2,4-dimethylphenol and 2,5-dimethylphenol into 3,5-dimethylcatechol and 3,6-dimethylcatechol, respectively, which, however, were not cleaved by catechol 2,3-dioxygenase. The identified gene cluster displayed a gene order similar to that of the Pseudomonas sp. strain CF600 dmp operon for phenol catabolism and was found to be coregulated by the tou operon activator TouR. A hypothesis about the evolution of the toluene and o-xylene catabolic pathway in P. stutzeri OX1 is discussed.

* Corresponding author. Mailing address: Dip. Genetica e Biologia dei Microrganismi, Via Celoria 26, 20133 Milano, Italy. Phone: 39-02.26605227. Fax: 39-02.2664551. E-mail: Paola.Barbieri{at}unimi.it.

Applied and Environmental Microbiology, July 2001, p. 3304-3308, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3304-3308.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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