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Applied and Environmental Microbiology, July 2001, p. 3314-3318, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3314-3318.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Congruent Phylogenies of Most Common Small-Subunit rRNA and Dissimilatory Sulfite Reductase Gene Sequences Retrieved from Estuarine Sediments

Catherine Joulian,* Niels B. Ramsing, and Kjeld Ingvorsen

Institute of Biological Sciences, Department of Microbial Ecology, Aarhus University, Ny Munkegade, DK-8000 Aarhus C, Denmark

Received 24 October 2000/Accepted 28 March 2001

The diversity of sulfate-reducing bacteria (SRB) in brackish sediment was investigated using small-subunit rRNA and dissimilatory sulfite reductase (DSR) gene clone libraries and cultivation. The phylogenetic affiliation of the most commonly retrieved clones for both genes was strikingly similar and produced Desulfosarcina variabilis-like sequences from the inoculum but Desulfomicrobium baculatum-like sequences from a high dilution in natural media. Related organisms were subsequently cultivated from the site. PCR bias appear to be limited (or very similar) for the two primersets and target genes. However, the DSR primers showed a much higher phylogenetic specificity. DSR gene analysis is thus a promising and specific approach for investigating SRB diversity in complex habitats.

* Corresponding author. Mailing address: Department of Microbial Ecology, Ny Munkegade, Building 540, DK-8000 Aarhus C, Denmark. Phone: 45-8942-3246. Fax: 45-8612-7191. E-mail: catherine.joulian{at}biology.aau.dk.

Applied and Environmental Microbiology, July 2001, p. 3314-3318, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3314-3318.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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