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Applied and Environmental Microbiology, August 2001, p. 3391-3395, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3391-3395.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Effects of Nondigestible Oligosaccharides on Salmonella enterica Serovar Typhimurium and Nonpathogenic Escherichia coli in the Pig Small Intestine In Vitro

Patrick J. Naughton,* Lene Lind Mikkelsen, and Bent Borg Jensen

Microbiology Section, Department of Animal Nutrition and Physiology, Ministry of Food, Agriculture and Fisheries, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK-8830 Tjele, Denmark

Received 29 January 2001/Accepted 16 May 2001

An in vitro intestinal tissue model was developed for the investigation of bacterial association in the pig small intestine under different dietary regimes. In preliminary experiments, jejunal and ileal tissue was taken from Danish Landrace pigs fed standard diet and inoculated with either Salmonella or nonpathogenic Escherichia coli strains. Higher numbers of salmonellae associated with the ileal tissues, but the numbers did not reach significance. Hence, jejunal sections were inoculated with nonpathogenic E. coli and ileal sections were inoculated with salmonellae in the presence of mannose or commercial nondigestible oligosaccharides (NDO) at 2.5%. There was a significant decrease in E. coli associated with the jejunum in the presence of mannose (P < 0.05). Furthermore, in pigs fed a diet supplemented with commercial NDO at 4% there was a significant reduction in the numbers of E. coli in jejunal organ cultures of pigs fed the FOS diet (P < 0.05). There was a reduction, though not a significant one, in the association of Salmonella sp. to the ileal sections of pigs fed the commercial FOS diet. The feeding of commercial GOS or its addition to organ cultures did not affect E. coli or Salmonella numbers.

* Corresponding author. Mailing address: School of Biomedical Sciences, University of Ulster, Cromore Rd, Coleraine, County Londonderry, Northern Ireland BT52 ISA, United Kingdom. Phone: 44-28-7032-4689. Fax: 44-28-7032-4965. E-mail: PJ.Naughton{at}ulst.ac.uk.

Applied and Environmental Microbiology, August 2001, p. 3391-3395, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3391-3395.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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