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Applied and Environmental Microbiology, August 2001, p. 3426-3433, Vol. 67, No. 8
Institute of Soil, Water and Environmental
Sciences, Agricultural Research Organization, The Volcani Research
Center, Bet-Dagan 50-250,1 and
Water-Soil-Environment, Agricultural Engineering, Technion
Institute, Haifa 32-000,2 Israel
Received 8 January 2001/Accepted 30 May 2001
The effect of effluent irrigation on community composition and
function of ammonia-oxidizing bacteria (AOB) in soil was evaluated, using techniques of molecular biology and analytical soil chemistry. Analyses were conducted on soil sampled from lysimeters and from a
grapefruit orchard which had been irrigated with wastewater effluent or
fertilizer-amended water (FAW). Specifically, comparisons of AOB
community composition were conducted using denaturing gradient gel
electrophoresis (DGGE) of PCR-amplified fragments of the gene encoding
the
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.8.3426-3433.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Influence of Effluent Irrigation on Community
Composition and Function of Ammonia-Oxidizing Bacteria in
Soil
-subunit of the ammonia monooxygenase gene (amoA) recovered from soil samples and subsequent sequencing of relevant bands. A significant and consistent shift in the population composition of AOB was detected in soil irrigated with effluent. This shift was
absent in soils irrigated with FAW, despite the fact that the ammonium
concentration in the FAW was similar. At the end of the irrigation
period, Nitrosospira-like populations were dominant in
soils irrigated with FAW, while Nitrosomonas-like
populations were dominant in effluent-irrigated soils. Furthermore,
DGGE analysis of the amoA gene proved to be a powerful tool
in evaluating the soil AOB community population and population shifts therein.
*
Corresponding author. Mailing address: Institute of
Soil, Water and Environmental Sciences, Agricultural Research
Organization (ARO), The Volcani Research Center, P.O. Box 06, Bet-Dagan
50-250, Israel. Phone: 972-3-9683316. Fax: 972-3-9604017. E-mail:
minz{at}volcani.agri.gov.il.
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