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Applied and Environmental Microbiology, August 2001, p. 3702-3706, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3702-3706.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of a Granulovirus Isolated from Epinotia aporema Wals. (Lepidoptera: Tortricidae) Larvae

Alicia Sciocco-Cap,1 Alejandro D. Parola,2,3 Alina V. Goldberg,1 Pablo D. Ghiringhelli,2 and Víctor Romanowski2,3,*

Instituto de Microbiología y Zoología Agrícola, CNIA-INTA, CC 25, 1712 Castelar1; Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, 1976 Bernal2; and Instituto de Bioquímica y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 1900 La Plata3, Argentina

Received 8 January 2001/Accepted 11 May 2001

A granulovirus (GV) isolated from Epinotia aporema (Lepidoptera: Tortricidae)---a major soybean pest---was studied in terms of its main morphological, biochemical, and biological properties. The ovoidal occlusion bodies were 466 by 296 nm in size, and their most prominent protein had an apparent molecular mass of 29 kDa. Its amino-terminal sequence was remarkably homologous to that of the granulins of other GVs. The DNA genome size was estimated to be 120 kbp. The high specificity and pathogenicity of this newly described granulovirus (EpapGV) indicate that it is indeed a good candidate for the biological control of this pest.

* Corresponding author. Mailing address: Instituto de Bioquímica y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, calle 49 y 115, 1900 La Plata, Argentina. Phone: 54-221-4250497, ext. 32. Fax: 54-221-4259223. E-mail: victor{at}biol.unlp.edu.ar.

Applied and Environmental Microbiology, August 2001, p. 3702-3706, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3702-3706.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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