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Applied and Environmental Microbiology, August 2001, p. 3728-3731, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3728-3731.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Isolation and Regulation of Sinorhizobium meliloti 1021 Loci Induced by Oxygen Limitation

Jodi R. Trzebiatowski,^1,* Daniel M. Ragatz,^1,2 and Frans J. de Bruijn^1,2,3,

MSU-DOE Plant Research Laboratory,¹ NSF Center for Microbial Ecology,² and Department of Microbiology,³ Michigan State University, East Lansing, Michigan 48824

Received 29 January 2001/Accepted 30 May 2001

Eleven Sinorhizobium meliloti 1021 loci whose expression was induced under low oxygen concentrations were identified in a collection of 5,000 strains carrying Tn5-1063 (luxAB) transcriptional reporter gene fusions. The 11 Tn5-1063-tagged loci were cloned and characterized. The dependence of the expression of the tagged loci on the FixL/FixJ oxygen-sensing two-component regulatory system was examined. Three of the loci were found to be dependent upon fixL and fixJ for their expression, while one locus showed a partial dependence. The remaining seven loci showed fixL- and fixJ-independent induction of expression in response to oxygen limitation. This suggests that in S. meliloti, additional regulatory system(s) exist that respond either directly or indirectly to oxygen limitation conditions.

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^* Corresponding author. Mailing address: Center for Molecular Medicine and Genetics, Wayne State University, 540 E. Canfield St., Detroit, MI 48201. Phone: (313) 577-1627. Fax: (313) 577-5218. E-mail: jparrish{at}genetics.wayne.edu.

Present address: Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, BP27, 31326 Castanet Tolosan Cedex, France.

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Applied and Environmental Microbiology, August 2001, p. 3728-3731, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3728-3731.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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