AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hirano, S. S.
Right arrow Articles by Upper, C. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hirano, S. S.
Right arrow Articles by Upper, C. D.
Agricola
Right arrow Articles by Hirano, S. S.
Right arrow Articles by Upper, C. D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2001, p. 3735-3738, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3735-3738.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Use of an Intergenic Region in Pseudomonas syringae pv. Syringae B728a for Site-Directed Genomic Marking of Bacterial Strains for Field Experiments

Susan S. Hirano,1,* David K. Willis,1,2 Murray K. Clayton,1,3 and Christen D. Upper1,2

Departments of Plant Pathology1 and Statistics,3 University of Wisconsin---Madison, and Plant Disease Resistance Research Unit, Agricultural Research Service, U.S. Department of Agriculture,2 Madison, Wisconsin 53706

Received 8 January 2001/Accepted 30 May 2001

To construct differentially-marked derivatives of our model wild-type strain, Pseudomonas syringae pv. syringae B728a (a causal agent of bacterial brown spot disease in snap bean plants), for field experiments, we selected a site in the gacS-cysM intergenic region for site-directed insertion of antibiotic resistance marker cassettes. In each of three field experiments, population sizes of the site-directed chromosomally marked B728a derivatives in association with snap bean plants were not significantly different from that of the wild-type strain. Inserts of up to 7 kb of DNA in the intergenic region did not measurably affect fitness of B728a in the field. The site is useful for site-directed genomic insertions of single copies of genes of interest.

* Corresponding author. Mailing address: Department of Plant Pathology, University of Wisconsin---Madison, 1630 Linden Drive, Madison, WI 53706. Phone: (608) 262-7236. Fax: (608) 263-2626. E-mail: ssh{at}plantpath.wisc.edu.

Applied and Environmental Microbiology, August 2001, p. 3735-3738, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3735-3738.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2001 by the American Society for Microbiology. All rights reserved.