This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Radnedge, L.
Right arrow Articles by Andersen, G. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Radnedge, L.
Right arrow Articles by Andersen, G. L.
Agricola
Right arrow Articles by Radnedge, L.
Right arrow Articles by Andersen, G. L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2001, p. 3759-3762, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3759-3762.2001

Identification of Nucleotide Sequences for the Specific and Rapid Detection of Yersinia pestis

Lyndsay Radnedge,1 Silvia Gamez-Chin,1 Paula M. McCready,1 Patricia L. Worsham,2 and Gary L. Andersen1,*

Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, California 94551,1 and United States Army Research Institute of Infectious Diseases, Fort Detrick, Maryland 217022

Received 1 March 2001/Accepted 7 May 2001

Suppression subtractive hybridization, a cost-effective approach for targeting unique DNA, was used to identify a 41.7-kb Yersinia pestis-specific region. One primer pair designed from this region amplified PCR products from natural isolates of Y. pestis and produced no false positives for near neighbors, an important criterion for unambiguous bacterial identification.

* Corresponding author. Mailing address: Biology and Biotechnology Research Program, L-441, 7000 East Avenue, Livermore, CA 94550. Phone: (925) 423-2525. Fax: (925) 422-2282. E-mail: Andersen2{at}LLNL.GOV.

Applied and Environmental Microbiology, August 2001, p. 3759-3762, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3759-3762.2001


This article has been cited by other articles:

  • Saxena, D., Caufield, P. W., Li, Y., Brown, S., Song, J., Norman, R. (2008). Genetic Classification of Severe Early Childhood Caries by Use of Subtracted DNA Fragments from Streptococcus mutans. J. Clin. Microbiol. 46: 2868-2873 [Abstract] [Full Text]  
  • Chain, P. S. G., Hu, P., Malfatti, S. A., Radnedge, L., Larimer, F., Vergez, L. M., Worsham, P., Chu, M. C., Andersen, G. L. (2006). Complete Genome Sequence of Yersinia pestis Strains Antiqua and Nepal516: Evidence of Gene Reduction in an Emerging Pathogen.. J. Bacteriol. 188: 4453-4463 [Abstract] [Full Text]  
  • Marenda, M. S., Sagne, E., Poumarat, F., Citti, C. (2005). Suppression subtractive hybridization as a basis to assess Mycoplasma agalactiae and Mycoplasma bovis genomic diversity and species-specific sequences. Microbiology 151: 475-489 [Abstract] [Full Text]  
  • Lehours, P, Dupouy, S, Bergey, B, Ruskone-Foumestraux, A, Delchier, J C, Rad, R, Richy, F, Tankovic, J, Zerbib, F, Megraud, F, Menard, A (2004). Identification of a genetic marker of Helicobacter pylori strains involved in gastric extranodal marginal zone B cell lymphoma of the MALT-type. Gut 53: 931-937 [Abstract] [Full Text]  
  • Anisimov, A. P., Lindler, L. E., Pier, G. B. (2004). Intraspecific Diversity of Yersinia pestis. Clin. Microbiol. Rev. 17: 434-464 [Abstract] [Full Text]  
  • Loiez, C., Herwegh, S., Wallet, F., Armand, S., Guinet, F., Courcol, R. J. (2003). Detection of Yersinia pestis in Sputum by Real-Time PCR. J. Clin. Microbiol. 41: 4873-4875 [Abstract] [Full Text]  
  • Radnedge, L., Agron, P. G., Hill, K. K., Jackson, P. J., Ticknor, L. O., Keim, P., Andersen, G. L. (2003). Genome Differences That Distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Appl. Environ. Microbiol. 69: 2755-2764 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»