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Applied and Environmental Microbiology, September 2001, p. 4024-4029, Vol. 67, No. 9
Marine Biology Research Division and Center
for Marine Biotechnology and Biomedicine, Scripps Institution of
Oceanography, University of California, San Diego, La Jolla, California
92093-0202
Received 8 February 2001/Accepted 23 June 2001
A yellow-pigmented marine bacterium, designated strain SD-21, was
isolated from surface sediments of San Diego Bay, San Diego, Calif.,
based on its ability to oxidize soluble Mn(II) to insoluble Mn(III, IV)
oxides. 16S rRNA analysis revealed that this organism was most closely
related to members of the genus Erythrobacter, aerobic
anoxygenic phototrophic bacteria within the
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.9.4024-4029.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Enzymatic Manganese(II) Oxidation by a Marine
-Proteobacterium

-4 subgroup of the
Proteobacteria (
-4 Proteobacteria).
SD-21, however, has a number of distinguishing phenotypic features
relative to Erythrobacter species, including the ability
to oxidize Mn(II). During the logarithmic phase of growth, this
organism produces Mn(II)-oxidizing factors of
250 and 150 kDa that
are heat labile and inhibited by both azide and
o-phenanthroline, suggesting the involvement of a
metalloenzyme. Although the expression of the Mn(II) oxidase was not
dependent on the presence of Mn(II), higher overall growth yields were
reached in cultures incubated with Mn(II) in the culture medium. In
addition, the rate of Mn(II) oxidation appeared to be slower in
cultures grown in the light. This is the first report of Mn(II)
oxidation within the
-4 Proteobacteria as well as the
first Mn(II)-oxidizing proteins identified in a marine gram-negative bacterium.
*
Corresponding author. Mailing address: Marine Biology
Research Division and Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San
Diego, 9500 Gilman Dr., La Jolla, California 92093-0202. Phone: (858)
534-5470. Fax: (858) 534-7313. E-mail: btebo{at}ucsd.edu.
Present address: Department of Geosciences, Princeton University,
Princeton, NJ 08544.
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