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Applied and Environmental Microbiology, September 2001, p. 4091-4095, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4091-4095.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Differentiation of Strains of Xylella fastidiosa by a Variable Number of Tandem Repeat Analysis

Helvécio Della Coletta-Filho,* Marco Aurélio Takita, Alessandra Alves de Souza, Carlos Ivan Aguilar-Vildoso, and Marcos Antonio Machado

Centro de Citricultura Sylvio Moreira, Instituto Agronômico, CEP 13490-970, Cordeirópolis, São Paulo, Brazil

Received 27 March 2001/Accepted 27 June 2001

Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen Xylella fastidiosa and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, which we examined for allelic polymorphisms using PCR. Five genuine VNTR loci were highly polymorphic within a set of 27 X. fastidiosa strains from different hosts. The highest average Nei's measure of genetic diversity (H) estimated for VNTR loci was 0.51, compared to 0.17 derived from randomly amplified polymorphic DNA (RAPD) analysis. For citrus X. fastidiosa strains, some specific VNTR loci had a H value of 0.83, while the maximum value given by specific RAPD loci was 0.12. Our approach using VNTR markers provides a high-resolution tool for epidemiological, genetic, and ecological analysis of citrus-specific X. fastidiosa strains.

* Corresponding author. Mailing address: Laboratório de Biotecnologia, Centro de Citricultura Sylvio Moreira, Instituto Agronômico, CEP 13490-970, CP04, Cordeirópolis, São Paulo, Brazil. Phone and fax: 19 546-1399. E-mail: helvecio{at}centrodecitricultura.br.

Applied and Environmental Microbiology, September 2001, p. 4091-4095, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4091-4095.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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