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Applied and Environmental Microbiology, September 2001, p. 4111-4118, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4111-4118.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Ruminococcin A, a New Lantibiotic Produced by a Ruminococcus gnavus Strain Isolated from Human Feces

J. Dabard,1 C. Bridonneau,1 C. Phillipe,1 P. Anglade,2 D. Molle,3 M. Nardi,2 M. Ladiré,1 H. Girardin,4 F. Marcille,1 A. Gomez,1 and M. Fons1,*

Unité d'Ecologie et de Physiologie du Système Digestif1 and Unité de Biochimie et Structure des Protéines,2 Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas Cedex, Laboratoire de Recherches de Technologie Laitière, Institut National de la Recherche Agronomique, 35042 Rennes Cedex,3 and Laboratoire de Microbiologie, Institut National de la Recherche Agronomique, 84914 Avignon Cedex 9,4 France

Received 17 April 2001/Accepted 21 June 2001

When cultivated in the presence of trypsin, the Ruminococcus gnavus E1 strain, isolated from a human fecal sample, was able to produce an antibacterial substance that accumulated in the supernatant. This substance, called ruminococcin A, was purified to homogeneity by reverse-phase chromatography. It was shown to be a 2,675-Da bacteriocin harboring a lanthionine structure. The utilization of Edman degradation and tandem mass spectrometry techniques, followed by DNA sequencing of part of the structural gene, allowed the identification of 21 amino acid residues. Similarity to other bacteriocins present in sequence libraries strongly suggested that ruminococcin A belonged to class IIA of the lantibiotics. The purified ruminococcin A was active against various pathogenic clostridia and bacteria phylogenetically related to R. gnavus. This is the first report on the characterization of a bacteriocin produced by a strictly anaerobic bacterium from human fecal microbiota.

* Corresponding author. Mailing address: Unité d'Ecologie et de Physiologie du Système Digestif, Bat 440---C.R.J., Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas Cedex, France. Phone and fax: 33 1 34 65 24 65. E-mail: fons{at}biotec.jouy.inra.fr.

Applied and Environmental Microbiology, September 2001, p. 4111-4118, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4111-4118.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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