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Applied and Environmental Microbiology, September 2001, p. 4279-4285, Vol. 67, No. 9
Departamento de Genética, Facultad de
Biología, Universidad de Sevilla, E-41080 Sevilla, Spain
Received 19 January 2001/Accepted 27 May 2001
We isolated spontaneous mutants from Saccharomyces
cerevisiae (baker's yeast V1) that were resistant to
2-deoxy-D-glucose and had improved fermentative capacity on
sweet doughs. Three mutants could grow at the same rate as the wild
type in minimal SD medium (0.17% Difco yeast nitrogen base without
amino acids and ammonium sulfate, 0.5% ammonium sulfate, 2%
glucose) and had stable elevated levels of maltase and/or
invertase under repression conditions but lower levels in
maltose-supplemented media. Two of the mutants also had high levels of
phosphatase active on 2-deoxy-D-glucose-6-phosphate. Dough fermentation (CO2 liberation) by two of the mutants
was faster and/or produced higher final volumes than that by the wild type, both under laboratory and industrial conditions, when the doughs
were supplemented with glucose or sucrose. However, the three mutants
were slower when fermenting plain doughs. Fermented sweet bakery
products obtained with these mutants were of better quality than those
produced by the wild type, with regard to their texture and their
organoleptic properties.
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.9.4279-4285.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Improved Properties of Baker's Yeast Mutants
Resistant to 2-Deoxy-D-Glucose
*
Corresponding author. Mailing address: Departamento de
Genética, Facultad de Biología, Universidad de Sevilla,
Apartado 1095, E-41080 Sevilla, Spain. Phone: 34.95.4557109. Fax:
34.95.4557104. E-mail: tahia{at}cica.es.
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