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Applied and Environmental Microbiology, September 2001, p. 4279-4285, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4279-4285.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Improved Properties of Baker's Yeast Mutants Resistant to 2-Deoxy-D-Glucose

Ana M. Rincón, Antonio C. Codón, Francisco Castrejón, and Tahía Benítez*

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, E-41080 Sevilla, Spain

Received 19 January 2001/Accepted 27 May 2001

We isolated spontaneous mutants from Saccharomyces cerevisiae (baker's yeast V1) that were resistant to 2-deoxy-D-glucose and had improved fermentative capacity on sweet doughs. Three mutants could grow at the same rate as the wild type in minimal SD medium (0.17% Difco yeast nitrogen base without amino acids and ammonium sulfate, 0.5% ammonium sulfate, 2% glucose) and had stable elevated levels of maltase and/or invertase under repression conditions but lower levels in maltose-supplemented media. Two of the mutants also had high levels of phosphatase active on 2-deoxy-D-glucose-6-phosphate. Dough fermentation (CO2 liberation) by two of the mutants was faster and/or produced higher final volumes than that by the wild type, both under laboratory and industrial conditions, when the doughs were supplemented with glucose or sucrose. However, the three mutants were slower when fermenting plain doughs. Fermented sweet bakery products obtained with these mutants were of better quality than those produced by the wild type, with regard to their texture and their organoleptic properties.


* Corresponding author. Mailing address: Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Apartado 1095, E-41080 Sevilla, Spain. Phone: 34.95.4557109. Fax: 34.95.4557104. E-mail: tahia{at}cica.es.


Applied and Environmental Microbiology, September 2001, p. 4279-4285, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4279-4285.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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