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Applied and Environmental Microbiology, September 2001, p. 4335-4337, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4335-4337.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Lytic and Lysogenic Infection of Diverse Escherichia coli and Shigella Strains with a Verocytotoxigenic Bacteriophage

Chloe E. James,1 Karen N. Stanley,2 Heather E. Allison,1 Harry J. Flint,2 Colin S. Stewart,2 Richard J. Sharp,3 Jon R. Saunders,1 and Alan J. McCarthy1,*

School of Biological Sciences, University of Liverpool, Liverpool, L69 7ZB,1 Gut Microbiology and Immunology Division, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB,2 and The Centre for Applied Microbiological Research, Porton Down, Salisbury,3 United Kingdom

Received 12 March 2001/Accepted 18 June 2001

A verocytotoxigenic bacteriophage isolated from a strain of enterohemorrhagic Escherichia coli O157, into which a kanamycin resistance gene (aph3) had been inserted to inactivate the verocytotoxin gene (vt2), was used to infect Enterobacteriaceae strains. A number of Shigella and E. coli strains were susceptible to lysogenic infection, and a smooth E. coli isolate (O107) was also susceptible to lytic infection. The lysogenized strains included different smooth E. coli serotypes of both human and animal origin, indicating that this bacteriophage has a substantial capacity to disseminate verocytotoxin genes. A novel indirect plaque assay utilizing an E. coli recA441 mutant in which phage-infected cells can enter only the lytic cycle, enabling detection of all infective phage, was developed.

* Corresponding author. Mailing address: School of Biological Sciences, Life Sciences Building, University of Liverpool, Crown Street, Liverpool L69 7ZB, United Kingdom. Phone: 44-151-794-4413. Fax: 44-151-794-4401. E-mail: aj55m{at}liverpool.ac.uk.

Applied and Environmental Microbiology, September 2001, p. 4335-4337, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4335-4337.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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