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Applied and Environmental Microbiology, September 2001, p. 4338-4341, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4338-4341.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Physiological Characterization of Pseudomonas putida DOT-T1E Tolerance to p-Hydroxybenzoate

María-Isabel Ramos-González,¹ Patricia Godoy,¹ Miguel Alaminos,¹ Arie Ben-Bassat,² and Juan-Luis Ramos^2,*

Department of Biochemistry and Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, E-18008 Granada, Spain,¹ and Central Research and Development, DuPont de Nemours, Newark, Delaware 19714-6101²

Received 26 February 2001/Accepted 4 June 2001

Pseudomonas putida DOT-T1E was isolated as a toluene-tolerant strain. We show that it is also able to grow on high concentrations (up to 17 g/liter [123 mM]) of p-hydroxybenzoate (4HBA). Tolerance to this aromatic carboxylic acid (up to 30 g/liter [217 mM]) is improved by preexposing the cells to low 4HBA concentrations; the adaptation process is caused by the substrate itself rather than by products resulting from its metabolism. The mechanisms of 4HBA tolerance seem to involve increased rigidity of the cell membrane as a result of a decrease in the cis/trans ratio of unsaturated fatty acids. In addition, energy-dependent efflux systems seem to operate in the exclusion of 4HBA from the cell membranes.

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^* Corresponding author. Mailing address: Estación Experimental del Zaidín, C/Prof. Albareda 1, E-18008 Granada, Spain. Phone: 34-958-121011. Fax: 34-958-129600. E-mail: jlramos{at}eez.csic.es.

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Applied and Environmental Microbiology, September 2001, p. 4338-4341, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4338-4341.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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