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Applied and Environmental Microbiology, September 2001, p. 4374-4376, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4374-4376.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Quantitative Comparisons of 16S rRNA Gene Sequence Libraries from Environmental Samples

David R. Singleton,1 Michelle A. Furlong,1 Stephen L. Rathbun,2 and William B. Whitman1,*

Departments of Microbiology1 and Statistics,2 University of Georgia, Athens, Georgia 30602-2605

Received 8 March 2001/Accepted 11 June 2001

To determine the significance of differences between clonal libraries of environmental rRNA gene sequences, differences between homologous coverage curves, CX(D), and heterologous coverage curves, CXY(D), were calculated by a Cramér-von Mises-type statistic and compared by a Monte Carlo test procedure. This method successfully distinguished rRNA gene sequence libraries from soil and bioreactors and correctly failed to find differences between libraries of the same composition.

* Corresponding author. Mailing address: Department of Microbiology, University of Georgia, 527 Biological Sciences Bldg.; Athens, GA 30602-2605. Phone: (706) 542-4219. Fax: (706) 542-2674. E-mail: whitman{at}arches.uga.edu.

Applied and Environmental Microbiology, September 2001, p. 4374-4376, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4374-4376.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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