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Applied and Environmental Microbiology, September 2001, p. 4377-4381, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4377-4381.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Dehalogenation of Chlorinated Hydroxybiphenyls by Fungal Laccase

Asgard Schultz,* Ulrike Jonas, Elke Hammer, and Frieder Schauer

Institute of Microbiology and Molecular Biology, Ernst Moritz Arndt University of Greifswald, D-17487 Greifswald, Germany

Received 27 February 2001/Accepted 26 June 2001

We have investigated the transformation of chlorinated hydroxybiphenyls by laccase produced by Pycnoporus cinnabarinus. The compounds used were transformed to sparingly water-soluble colored precipitates which were identified by gas chromatography-mass spectrometry as oligomerization products of the chlorinated hydroxybiphenyls. During oligomerization of 2-hydroxy-5-chlorobiphenyl and 3-chloro-4-hydroxybiphenyl, dechlorinated C---C-linked dimers were formed, demonstrating the dehalogenation ability of laccase. In addition to these nonhalogenated dimers, both monohalogenated and dihalogenated dimers were identified.


* Corresponding author: Mailing address: Institute of Microbiology and Molecular Biology, Ernst Moritz Arndt University of Greifswald, Friedrich-Ludwig-Jahn-Str. 15, D-17487 Greifswald, Germany. Phone: 49-(0)3834-864225. Fax: 49-(0)3834-864202. E-mail: asgard.schultz{at}web.de.


Applied and Environmental Microbiology, September 2001, p. 4377-4381, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4377-4381.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.