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Applied and Environmental Microbiology, September 2001, p. 4382-4384, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4382-4384.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Confirmation of Clostridium perfringens by Using Chromogenic and Fluorogenic Substrates

Philip W. Adcock and Christopher P. Saint*

Microbiology Unit, Australian Water Quality Centre, SA Water Corporation, Salisbury, South Australia 5108, Australia

Received 20 March 2001/Accepted 2 July 2001

The use of 4-methylumbelliferyl phosphate (MUP) and ortho-nitrophenyl-beta -D-galactopyranoside (ONPG) for the identification of Clostridium perfringens was investigated. A liquid assay containing both MUP and ONPG was a highly specific alternative method for C. perfringens confirmation, reducing incubation time from 48 to only 4 h. The assay solution is easy to prepare, does not require anaerobic conditions for use, and has an extended shelf life.

* Corresponding author. Mailing address: Australian Water Quality Centre, SA Water Corporation, Private Mail Bag 3, Salisbury, South Australia 5108, Australia. Phone: 618 8259 0331. Fax: 618 8259 0228. E-mail: chris.saint{at}sawater.sa.gov.au.

Applied and Environmental Microbiology, September 2001, p. 4382-4384, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4382-4384.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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