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Applied and Environmental Microbiology, January 2002, p. 102-105, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.102-105.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Isolation and Initial Characterization of a Bacterial Consortium Able To Mineralize Fluorobenzene

M.F. Carvalho,1 C.C.T. Alves,1 M.I.M. Ferreira,1 P. De Marco,2 and P.M.L. Castro1*

Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072 Porto,1 IBMC, Universidade do Porto, 4150-180 Porto, Portugal2

Received 13 July 2000/ Accepted 24 October 2001

Fluorinated compounds are known to be more resistant to microbial degradation than other halogenated chemicals. A microbial consortium capable of aerobic biodegradation of fluorobenzene (FB) as the sole source of carbon and energy was isolated by selective enrichment from sediments collected in a drain near an industrial site. A combination of three microbial strains recovered from the enriched consortium was shown to be necessary for complete FB mineralization. Two of the strains (F1 and F3) were classified by 16S rRNA analysis as belonging to the Sphingobacterium/Flavobacterium group, while the third (F4) falls in the ß-Proteobacteria group, clustering with Alcaligenes species. Strain F4 was consistently found in the liquid cultures in a much greater proportion than strains F1 and F3 (86:8:6 for F4, F1, and F3, respectively). Stoichiometric release of fluoride ions was measured in batch and fed-batch cultures. In batch cultures, the consortium was able to use FB up to concentrations of 400 mg liter–1 and was able to utilize a range of other organic compounds, including 4-fluorophenol and 4-fluorobenzoate. To our knowledge this is the first time biodegradation of FB as a sole carbon source has been reported.

* Corresponding author. Mailing address: Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal. Phone: 351 22 5580059. Fax: 351 22 5090351. Email: plc{at}esb.ucp.pt.

Applied and Environmental Microbiology, January 2002, p. 102-105, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.102-105.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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