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Applied and Environmental Microbiology, January 2002, p. 37-45, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.37-45.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differentiation of Phytophthora infestans Sporangia from Other Airborne Biological Particles by Flow Cytometry

Jennifer P. Day, Douglas B. Kell, and Gareth W. Griffith*

Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, Wales SY23 3DA, United Kingdom

Received 17 April 2001/ Accepted 11 October 2001

The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or Botrytis spp., rust urediniospores, and pollen of grasses and plantain. Differentiation between P. infestans sporangia and powdery mildew conidia was not possible by these two methods but, when combined with analytical rules evolved by genetic programming methods, could be achieved after staining with the fluorescent brightener Calcofluor white M2R. The potential application of these techniques to the prediction of late-blight epiphytotics in the field is discussed.

* Corresponding author. Mailing address: Institute of Biological Sciences, Edward Llwyd Building, University of Wales, Aberystwyth, Ceredigion, Wales SY23 3DA, United Kingdom. Phone: 0044-1970-622325. Fax: 0044-1970-622350. E-mail: gwg{at}aber.ac.uk.

Applied and Environmental Microbiology, January 2002, p. 37-45, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.37-45.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Prigione, V., Lingua, G., Marchisio, V. F. (2004). Development and Use of Flow Cytometry for Detection of Airborne Fungi. Appl. Environ. Microbiol. 70: 1360-1365 [Abstract] [Full Text]  


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