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Applied and Environmental Microbiology, January 2002, p. 372-378, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.372-378.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of a Putative Transcriptional Regulator Controlling the Expression of Fouling Inhibitors in Pseudoalteromonas tunicata

Suhelen Egan,1,2 Sally James,1,2 and Staffan Kjelleberg1,2*

School of Microbiology and Immunology,1 Centre for Marine Biofouling and Bio-Innovation, University of New South Wales, Sydney 2052, New South Wales, Australia2

Received 1 August 2001/ Accepted 12 October 2001

The dark green pigmented marine bacterium Pseudoalteromonas tunicata colonizes living surfaces and produces a range of extracellular compounds that inhibit common fouling organisms, including marine invertebrate larvae, algae, bacteria, and fungi. We have observed a positive correlation between the antifouling activity of P. tunicata strain D2 and the expression of pigmentation. To address the hypothesis that pigmentation and antifouling may be jointly regulated in this organism and to begin to identify potential regulatory elements, we used transposon mutagenesis to generate a strain of P. tunicata deficient in antifouling activity. The data presented here describe the phenotypic and molecular characterization of a nonpigmented transposon mutant strain of P. tunicata (D2W2). Analyses of the antifouling capabilities of D2W2 demonstrate that this strain is deficient in the ability to inhibit each of the target fouling organisms. Genetic analysis of D2W2 identified a gene, designated wmpR (white mutant phenotype), with high sequence similarity to transcriptional regulators ToxR from Vibrio cholerae and CadC from Escherichia coli. Two-dimensional polyacrylamide gel electrophoresis analysis revealed that WmpR is essential for the expression of a significant subset of stationary-phase-induced proteins likely to be important for the synthesis of fouling inhibitors. The identification of a gene involved in the regulation of expression of antifouling phenotypes will contribute to the understanding of the interactions between bacteria and other surface-colonizing organisms in the marine environment.

* Corresponding author. Mailing address: School of Microbiology and Immunology, The University of New South Wales, Sydney 2052, New South Wales, Australia. Phone: (61) 2 93852102. Fax: (61) 2 93851591. E-mail: S.Kjelleberg{at}unsw.edu.au.

Applied and Environmental Microbiology, January 2002, p. 372-378, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.372-378.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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