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Applied and Environmental Microbiology, January 2002, p. 417-422, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.417-422.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Isolation of Gemmata-Like and Isosphaera-Like Planctomycete Bacteria from Soil and Freshwater

Jenny Wang,¹ Cheryl Jenkins,^1, Richard I. Webb,^1,2 and John A. Fuerst^1*

Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences,¹ Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Queensland 4072, Australia²

Received 2 July 2001/ Accepted 15 October 2001

New cultured strains of the planctomycete division (order Planctomycetales) of the domain Bacteria related to species in the genera Gemmata and Isosphaera were isolated from soil, freshwater, and a laboratory ampicillin solution. Phylogenetic analysis of the 16S rRNA gene from eight representative isolates showed that all the isolates were members of the planctomycete division. Six isolates clustered with Gemmata obscuriglobus and related strains, while two isolates clustered with Isosphaera pallida. A double-membrane-bounded nucleoid was observed in Gemmata-related isolates but not in Isosphaera-related isolates, consistent with the ultrastructures of existing species of each genus. Two isolates from this study represent the first planctomycetes successfully cultivated from soil.

Present address: Department of Microbiology, University of Washington, Seattle, WA 98195-7242.

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