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Applied and Environmental Microbiology, January 2002, p. 423-426, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.423-426.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of a Sinorhizobium Isolate and Its Extracellular Polymer Implicated in Pollutant Transport in Soil

J. Janecka,1,{dagger} M. B. Jenkins,1* N. S. Brackett,2 L. W. Lion,3 and W. C. Ghiorse1

Department of Microbiology,1 Department ofChemistry,2 School of Civil and Environmental Engineering, Cornell University, Ithaca, New York 148533

Received 28 June 2001/ Accepted 22 October 2001

A bacterium isolated from soil (designated 9702-M4) synthesizes an extracellular polymer that facilitates the transport of such hydrophobic pollutants as polynuclear aromatic hydrocarbons, as well as the toxic metals lead and cadmium in soil. Biolog analysis, growth rate determinations, and percent G+C content identify 9702-M4 as a strain of Sinorhizobium meliloti. Sequence analysis of a 16S rDNA fragment gives 9702-M4 a phylogenetic designation most closely related to Sinorhizobium fredii. The extracellular polymer of isolate 9702-M4 is composed of both an extracellular polysaccharide (EPS) and a rough lipopolysaccharide. The EPS component is composed mainly of 4-glucose linkages with monomers of galactose, mannose, and glucuronic acid and has pyruval and acetyl constituents. The lipid fraction and the negative charge associated with carbonyl groups of the exopolymer are thought to account for the binding of polynuclear aromatic hydrocarbons and cationic metals.

* Corresponding author. Present address: USDA-ARS, 1420 Experiment Station Rd., Watkinsville, GA 30677. Phone: (706) 769-5631. Fax: (706) 769-8962. E-mail: mjenkins{at}arches.uga.edu.

{dagger} Present address: School of Natural Resources, University of Vermont, Burlington, VT 05401.

Applied and Environmental Microbiology, January 2002, p. 423-426, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.423-426.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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