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Applied and Environmental Microbiology, January 2002, p. 427-429, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.427-429.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cryptosporidium parvum Mixed Genotypes Detected by PCR-Restriction Fragment Length Polymorphism Analysis

Carrie Reed, Gregory D. Sturbaum, Paul J. Hoover, and Charles R. Sterling*

Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721

Received 13 June 2001/ Accepted 22 October 2001

Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.

* Corresponding author. Mailing address: University of Arizona, Department of Veterinary Science and Microbiology, Bldg. LDG 90, 1117 E. Lowell St., Tucson, AZ 85721. Phone: (520) 621-4580. Fax: (520) 621-3588. E-mail: csterlin{at}u.arizona.edu.

Applied and Environmental Microbiology, January 2002, p. 427-429, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.427-429.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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