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Applied and Environmental Microbiology, October 2002, p. 4740-4750, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.4740-4750.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Relationship between Bacterial Community Composition and Bottom-Up versus Top-Down Variables in Four Eutrophic Shallow Lakes

Koenraad Muylaert,1* Katleen Van der Gucht,1,2 Nele Vloemans,1,2 Luc De Meester,3 Moniek Gillis,2 and Wim Vyverman1

Department of Biology,1 Department of Microbiology, University Ghent, 9000 Ghent,2 Laboratory of Aquatic Ecology, KULeuven, 3000 Leuven, Belgium3

Received 4 February 2002/ Accepted 17 July 2002

Bacterial community composition was monitored in four shallow eutrophic lakes during one year using denaturing gradient gel electrophoresis (DGGE) of PCR-amplified prokaryotic rDNA genes. Of the four lakes investigated, two were of the clearwater type and had dense stands of submerged macrophytes while two others were of the turbid type characterized by the occurrence of phytoplankton blooms. One turbid and one clearwater lake had high nutrient levels (total phosphorus, >100 µg liter-1) while the other lakes had relatively low nutrient levels (total phosphorus, <100 µg liter-1). For each lake, seasonal changes in the bacterial community were related to bottom-up (resources) and top-down (grazers) variables by using canonical correspondence analysis (CCA). Using an artificial model dataset to which potential sources of error associated with the use of relative band intensities in DGGE analysis were added, we found that preferential amplification of certain rDNA genes over others does not obscure the relationship between bacterial community composition and explanatory variables. Besides, using this artificial dataset as well as our own data, we found a better correlation between bacterial community composition and explanatory variables by using relative band intensities compared to using presence/absence data. While bacterial community composition was related to phytoplankton biomass in the high-nutrient lakes no such relation was found in the low-nutrient lakes, where the bacterial community is probably dependent on other organic matter sources. We used variation partitioning to evaluate top-down regulation of bacterial community composition after bottom-up regulation has been accounted for. Using this approach, we found no evidence for top-down regulation of bacterial community composition in the turbid lakes, while grazing by ciliates and daphnids (Daphnia and Ceriodaphnia) was significantly related to changes in the bacterial community in the clearwater lakes. Our results suggest that in eutrophic shallow lakes, seasonality of bacterial community structure is dependent on the dominant substrate source as well as on the food web structure.

* Corresponding author. Mailing address: Department of Biology, Universiteit Ghent, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium. Phone: 32 9 264 53 66. Fax: 32 9 264 53 34. E-mail: koenraad.muylaert{at}rug.ac.be.

Applied and Environmental Microbiology, October 2002, p. 4740-4750, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.4740-4750.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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