This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by La Ragione, R. M.
Right arrow Articles by Woodward, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by La Ragione, R. M.
Right arrow Articles by Woodward, M. J.
Agricola
Right arrow Articles by La Ragione, R. M.
Right arrow Articles by Woodward, M. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2002, p. 4932-4942, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.4932-4942.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Phenotypic and Genotypic Characterization of Avian Escherichia coli O86:K61 Isolates Possessing a Gamma-Like Intimin

R. M. La Ragione,1 I. M. McLaren,1 G. Foster,2 W. A. Cooley,1 and M. J. Woodward1*

Department of Bacterial Diseases, VLA (Weybridge), New Haw, Addlestone, Surrey KT15 3NB,1 SAC Veterinary Science Division, Drummondhill, Inverness IV2 4JZ, United Kingdom2

Received 28 November 2001/ Accepted 5 July 2002

Escherichia coli O86:K61 has long been associated with outbreaks of infantile diarrhea in humans and with diarrheal disease in many animal species. Studies in the late 1990s identified E. coli O86:K61 as the cause of mortality in a variety of wild birds, and in this study, 34 E. coli O86:K61 isolates were examined. All of the isolates were nonmotile, but most elaborated at least two morphologically distinct surface appendages that were confirmed to be type 1 and curli fimbriae. Thirty-three isolates were positive for the eaeA gene encoding a gamma type of intimin. No phenotypic or genotypic evidence was obtained for elaboration of Shiga-like toxins, but most isolates possessed the gene coding for the cytolethal distending toxin. Five isolates were selected for adherence assays performed with tissue explants and HEp-2 cells, and four of these strains produced attaching and effacing lesions on HEp-2 cells and invaded the cells, as determined by transmission electron microscopy. Two of the five isolates were inoculated orally into 1-day-old specific-pathogen-free chicks, and both of these isolates colonized, invaded, and persisted well in this model. Neither isolate produced attaching and effacing lesions in chicks, although some pathology was evident in the alimentary tract. No deaths were recorded in inoculated chicks. These findings are discussed in light of the possibility that wild birds are potential zoonotic reservoirs of attaching and effacing E. coli.

* Corresponding author. Mailing address: Department of Bacterial Diseases, VLA (Weybridge), New Haw, Woodham Lane, Addlestone, Surrey KT15 3NB, United Kingdom. Phone: 01932 357582. Fax: 01932 347046. E-mail: M.J.Woodward{at}vla.defra.gsi.gov.uk.

Applied and Environmental Microbiology, October 2002, p. 4932-4942, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.4932-4942.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Aktan, I., Carter, B., Wilking, H., La Ragione, R. M., Wieler, L., Woodward, M. J., Anjum, M. F. (2007). Influence of geographical origin, host animal and stx gene on the virulence characteristics of Escherichia coli O26 strains. J Med Microbiol 56: 1431-1439 [Abstract] [Full Text]  
  • Aktan, I., La Ragione, R. M., Pritchard, G. C., Woodward, M. J. (2007). Prevalence of attaching and effacing Escherichia coli serogroup O103 in orphan lambs on an open farm in eastern England. Vet Rec. 161: 386-387 [Full Text]  
  • Best, A., La Ragione, R. M., Sayers, A. R., Woodward, M. J. (2005). Role for Flagella but Not Intimin in the Persistent Infection of the Gastrointestinal Tissues of Specific-Pathogen-Free Chicks by Shiga Toxin-Negative Escherichia coli O157:H7. Infect. Immun. 73: 1836-1846 [Abstract] [Full Text]  
  • Anjum, M. F., Lucchini, S., Thompson, A., Hinton, J. C. D., Woodward, M. J. (2003). Comparative Genomic Indexing Reveals the Phylogenomics of Escherichia coli Pathogens. Infect. Immun. 71: 4674-4683 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»