Previous Article | Next Article 
Applied and Environmental Microbiology, October 2002, p. 5005-5011, Vol. 68, No. 10
0099-2240/02/$04.00+0 DOI: 10.1128/AEM.68.10.5005-5011.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
Widespread and Persistent Populations of a Major New Marine Actinomycete Taxon in Ocean Sediments
Tracy J. Mincer, Paul R. Jensen, Christopher A. Kauffman, and William Fenical*
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California 92093-0204
Received 12 March 2002/
Accepted 12 July 2002
A major taxon of obligate marine bacteria within the order Actinomycetales has been discovered from ocean sediments. Populations of these bacteria (designated MAR 1) are persistent and widespread, spanning at least three distinct ocean systems. In this study, 212 actinomycete isolates possessing MAR 1 morphologies were examined and all but two displayed an obligate requirement of seawater for growth. Forty-five of these isolates, representing all observed seawater-requiring morphotypes, were partially sequenced and found to share characteristic small-subunit rRNA signature nucleotides between positions 207 and 468 (Escherichia coli numbering). Phylogenetic characterization of seven representative isolates based on almost complete sequences of genes encoding 16S rRNA (16S ribosomal DNA) yielded a monophyletic clade within the family Micromonosporaceae and suggests novelty at the genus level. This is the first evidence for the existence of widespread populations of obligate marine actinomycetes. Organic extracts from cultured members of this new group exhibit remarkable biological activity, suggesting that they represent a prolific resource for biotechnological applications.
* Corresponding author. Mailing address: Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093-0204. Phone: (858) 534-2133. Fax: (858) 558-3702. E-mail: wfenical{at}ucsd.edu.
Applied and Environmental Microbiology, October 2002, p. 5005-5011, Vol. 68, No. 10
0099-2240/02/$04.00+0 DOI: 10.1128/AEM.68.10.5005-5011.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Pimentel-Elardo, S. M., Tiro, L. P., Grozdanov, L., Hentschel, U.
(2008). Saccharopolyspora cebuensis sp. nov., a novel actinomycete isolated from a Philippine sponge (Porifera). Int. J. Syst. Evol. Microbiol.
58: 628-632
[Abstract]
[Full Text]
-
Hozzein, W. N., Goodfellow, M.
(2007). Streptomyces synnematoformans sp. nov., a novel actinomycete isolated from a sand dune soil in Egypt. Int. J. Syst. Evol. Microbiol.
57: 2009-2013
[Abstract]
[Full Text]
-
Kasai, H., Katsuta, A., Sekiguchi, H., Matsuda, S., Adachi, K., Shindo, K., Yoon, J., Yokota, A., Shizuri, Y.
(2007). Rubritalea squalenifaciens sp. nov., a squalene-producing marine bacterium belonging to subdivision 1 of the phylum 'Verrucomicrobia'. Int. J. Syst. Evol. Microbiol.
57: 1630-1634
[Abstract]
[Full Text]
-
Gontang, E. A., Fenical, W., Jensen, P. R.
(2007). Phylogenetic Diversity of Gram-Positive Bacteria Cultured from Marine Sediments. Appl. Environ. Microbiol.
73: 3272-3282
[Abstract]
[Full Text]
-
Jensen, P. R., Williams, P. G., Oh, D.-C., Zeigler, L., Fenical, W.
(2007). Species-Specific Secondary Metabolite Production in Marine Actinomycetes of the Genus Salinispora. Appl. Environ. Microbiol.
73: 1146-1152
[Abstract]
[Full Text]
-
Scheuermayer, M., Gulder, T. A. M., Bringmann, G., Hentschel, U.
(2006). Rubritalea marina gen. nov., sp. nov., a marine representative of the phylum 'Verrucomicrobia', isolated from a sponge (Porifera).. Int. J. Syst. Evol. Microbiol.
56: 2119-2124
[Abstract]
[Full Text]
-
Kim, T. K., Hewavitharana, A. K., Shaw, P. N., Fuerst, J. A.
(2006). Discovery of a new source of rifamycin antibiotics in marine sponge actinobacteria by phylogenetic prediction.. Appl. Environ. Microbiol.
72: 2118-2125
[Abstract]
[Full Text]
-
Busti, E., Monciardini, P., Cavaletti, L., Bamonte, R., Lazzarini, A., Sosio, M., Donadio, S.
(2006). Antibiotic-producing ability by representatives of a newly discovered lineage of actinomycetes.. Microbiology
152: 675-683
[Abstract]
[Full Text]
-
Mincer, T. J., Fenical, W., Jensen, P. R.
(2005). Culture-Dependent and Culture-Independent Diversity within the Obligate Marine Actinomycete Genus Salinispora. Appl. Environ. Microbiol.
71: 7019-7028
[Abstract]
[Full Text]
-
Maldonado, L. A., Fenical, W., Jensen, P. R., Kauffman, C. A., Mincer, T. J., Ward, A. C., Bull, A. T., Goodfellow, M.
(2005). Salinispora arenicola gen. nov., sp. nov. and Salinispora tropica sp. nov., obligate marine actinomycetes belonging to the family Micromonosporaceae. Int. J. Syst. Evol. Microbiol.
55: 1759-1766
[Abstract]
[Full Text]
-
Magarvey, N. A., Keller, J. M., Bernan, V., Dworkin, M., Sherman, D. H.
(2004). Isolation and Characterization of Novel Marine-Derived Actinomycete Taxa Rich in Bioactive Metabolites. Appl. Environ. Microbiol.
70: 7520-7529
[Abstract]
[Full Text]
-
Challis, G. L., Hopwood, D. A.
(2003). Synergy and contingency as driving forces for the evolution of multiple secondary metabolite production by Streptomyces species. Proc. Natl. Acad. Sci. USA
100: 14555-14561
[Abstract]
[Full Text]
-
Bruns, A., Philipp, H., Cypionka, H., Brinkhoff, T.
(2003). Aeromicrobium marinum sp. nov., an abundant pelagic bacterium isolated from the German Wadden Sea. Int. J. Syst. Evol. Microbiol.
53: 1917-1923
[Abstract]
[Full Text]
-
Stach, J. E. M., Maldonado, L. A., Masson, D. G., Ward, A. C., Goodfellow, M., Bull, A. T.
(2003). Statistical Approaches for Estimating Actinobacterial Diversity in Marine Sediments. Appl. Environ. Microbiol.
69: 6189-6200
[Abstract]
[Full Text]
Copyright © 2002 by the American Society for Microbiology. All rights reserved.